| 李 艳，王 磊，蔡汝健，蒋智勇，勾红潮，楚品品，宋 帅，李春玲.猪葡萄球菌感染BALB/c小鼠血清中细胞因子表达的抗体芯片分析[J].广东农业科学,2018,45(8):113-117 |
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猪葡萄球菌感染BALB/c小鼠血清中细胞因子表达的抗体芯片分析 |
| Expression of serum cytokines in BALB/c mice with Staphylococcus hyicus detected by antibody chip |
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| DOI：10.16768/j.issn.1004-874X.2018.08.017 |
| 中文关键词: 抗体芯片 猪葡萄球菌 炎性细胞因子 分子标记物 |
| 英文关键词: antibody chip Staphylococcus hyicus inflammatory cytokine molecular marker sequencing |
| 基金项目:广东省科技计划项目（2017A030303043，2015B020230004） |
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| 摘要点击次数: 1608 |
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| 中文摘要: |
| 为分析猪葡萄球菌感染BALB/c 小鼠不同时间点血清中炎性细胞因子表达的变化，收集猪葡
萄球菌感染BALB/c 小鼠不同感染时段（24、48 h）的血清，采用细胞因子抗体芯片技术检测32 种细胞因
子水平。结果显示，猪葡萄球菌感染小鼠后血清细胞因子表达水平发生了明显变化：与对照相比，感染后
24 h，血清细胞因子中有5 种细胞因子表达出现了显著性变化，其中表达升高2 倍以上的细胞因子有4 种
（G-CSF、IL-6、KC、MCP-5）；感染后48 h，血清细胞因子中共有4 种细胞因子表达出现了显著性变化，
其中表达升高2 倍以上的细胞因子有3 种（G-CSF、KC、MCP-5）；感染24 h 和48 h 后IL-12 的表达均
显著低于对照。炎性细胞因子在猪葡萄球菌感染中发挥重要作用，利用细胞因子抗体芯片从血清中筛选猪
葡萄球菌相关蛋白分子标记物是可行的。 |
| 英文摘要: |
| In order to analyze the expression of inflammatory cytokines in serum of BALB/c mice infected with
Staphylococcus hyicus at different time point, the serum of the BALB/c mice infected with staphylococcus hyicus were
collected at both 24h and 48h post infection, and the levels of 32 cytokines were detected by cytokine antibody chip
technology. The results showed that there was a significant change in the level of cytokine from serum in mice infected
with Staphylococcus hyicus. Compared with the control group, there were 5 cytokines significantly changed 24h post
infection. Among them, 4 cytokines (G-CSF, IL-6, KC, MCP-5) were expressed 2 times greater than controls. After
infected 48h, 4 cytokines significantly changed, and 3 of them, including G-CSF, KC, MCP-5, were expressed 2 times
greater than the control levels. The expression level of IL-12 were significantly lower than the control levels at both
24h and 48h after infection. The inflammatory cytokines play an important role in the process of S. hyicus infection. It
is feasible to screen staphylococcus hyicus related protein biomarkers from serum by applying cytokine antibody array. |
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