| 李施蒙.芸薹属AC 基因组MTP1 基因的生物信息学分析[J].广东农业科学,2018,45(9):21-27 |
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芸薹属AC 基因组MTP1 基因的生物信息学分析 |
| Bioinformatics analysis on MTP1 genes in the AC genomes of Brassicas |
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| DOI：10.16768/j.issn.1004-874X.2018.09.004 |
| 中文关键词: 甘蓝型油菜 MTP1 基因 重金属耐受蛋白 锌 CDF 家族 生物信息学 |
| 英文关键词: Brassica napus L. MTP1 gene metal tolerance protein Zn cation diffuse facilitator proteins
family bioinformatics |
| 基金项目:西藏自治区农牧科学院农业研究所青年基金（2017NKS-QNJJ） |
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| 中文摘要: |
| 以拟南芥（Arabidopsis thaliana）AtMTP1 基因序列为参考，利用白菜（Brassica rapa）、甘
蓝（Brassica oleracea）、甘蓝型油菜（Brassica napus）全基因组数据对MTP1 基因进行全基因组水平鉴定，
通过Blastn 比对和保守结构域分析，在芸薹属AC 基因组中共鉴定到8 个MTP1 基因，其中白菜1 个、甘
蓝3 个、甘蓝型油菜4 个（A 亚基因组3 个、C 亚基因组1 个）。生物信息学分析结果表明，在蛋白质理
化性质、二级结构、跨膜结构域、磷酸化位点等方面，3 个物种中的MTP1 基因间各有差异，并使用甘蓝
型油菜转录组数据比较分析4 个BnMTP1 基因在不同组织部位的表达模式。 |
| 英文摘要: |
| Genome databases of Brassica rapa, Brassica oleracea and Brassica napus were used to identify the
MTP1 genes in the whole genomes, the AtMTP1 gene sequences in Arabidopsis thaliana were used as reference. Blastn
alignment and conserved domain analysis were performed against the AC genomes in Brassicas, and 8 MTP1 genes were
found, in which 1, 3 and 4 homologous sequences of MTP1 genes in B. rapa, B. oleracea and B. napus (3 genes in A subgenome,
1 gene in C subgenome), respectively. Bioinformatics analysis by bioinformatics tools and resources from the
internet of MTP1 genes in Brassicas showed that, different MTP1 genes of the 3 species were different in the composition
of nucleic acid sequences and amino acid sequences, subcellular localization, phosphorylation sites, hydrophilicity and
hydrophobicity, secondary structure and conserved domains. The expression patterns of 4 BnMTP1 genes in different
tissues were compared and analyzed with the transcriptome data of Brassica napus. |
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