| 向 蓉，翟少伦，王晓虎，陈 晶，黄 元，黄 忠，向 华.羊口疮病毒ORFV/GD-QY/01 株的分离鉴定[J].广东农业科学,2018,45(9):116-120 |
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羊口疮病毒ORFV/GD-QY/01 株的分离鉴定 |
| Establishment of a quantitative prediction model for mirror carp freshness by near infrared spectroscopy |
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| DOI：10.16768/j.issn.1004-874X.2018.09.019 |
| 中文关键词: 羊口疮病毒 分离鉴定 B2L 基因 诊断 遗传进化分析 |
| 英文关键词: Orf virus isolation and identification B2L gene diagnose phylogenetic analysis |
| 基金项目:国家重点研发计划项目（2016YFD0501505）；广东省科技计划项目（2015A010107009，
2015B020203005，2017A040405037）；广东省农业科学院院长基金（201624）；广东省农业科学院培育学科建设
（牛羊疫病防控团队）项目 |
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| 中文摘要: |
| 利用MDBK 细胞对广东清远某羊场的痂皮病例进行病毒分离，获得一株ORFV，命名为
ORFV/GD-QY/01。参考NCBI 羊口疮病毒（Orf virus）保守基因B2L 序列，设计一对特异性引物进行PCR
扩增后测序；运用序列分析软件对获得的羊口疮病毒株B2L 基因核苷酸序列与其他羊口疮毒株进行多序列
比对，构建系统发育进化树。结果表明：接种病料悬液的MDBK 细胞出现细胞病变，扩增出ORFV B2L 基
因，该毒株与台湾山羊株（EU935106、DQ904351）相似性最高（均为99.2%），亲缘关系最近。 |
| 英文摘要: |
| Virus was isolated from scab materials of lambs from a goatfarm in Qingyuan city of Guangdong
province with clinical sore mouth symptom by passaging in MDBK cells, A strain of Orf virus was successfully
isolated and identified, named ORFV/GD-QY/01. A pair of specific primers were designed according to B2L
gene sequence in NCBI, and PCR method was used to amplify the B2L gene. Phylogenetic analysis of major
envelope B2L gene of parapoxvirus with DNAStar. The result showed that MDBK cell cultures infected with crust
materials displayed cytopathic effects, B2L gene was amplified，the isolated strain was closest to similarity of
nucleotide sequences（99.2%） with those of Taiwan strain（EU93510 6, DQ904351）. |
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