| 李 亚 1，2，余沁涵 1，鲍敏丽 2，陈燕玲 2，邓晓玲 2.黄龙病菌感染对长春花 SOD 和 POD 活性的影响[J].广东农业科学,2019,46(1):71-78 |
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黄龙病菌感染对长春花 SOD 和 POD 活性的影响 |
| Effect of Candidatus Liberibacter asiaticus Infection on the Activities of SOD and POD in Catharanthus roseus |
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| DOI：10.16768/j.issn.1004-874X.2019.01.011 |
| 中文关键词: 长春花 黄龙病菌 超氧化物岐化酶（SOD） 过氧化物酶（POD） 酶活力 |
| 英文关键词: Catharanthus roseus Candidatus Liberibacter asiaticus(CaLas) superoxide dismutase(SOD) peroxidase(POD) enzyme activity |
| 基金项目:国家现代农业（柑桔）产业技术体系（CARS-26）专项经费；广东海洋大学博士启动项目（R17060）；广东海洋大学校选课题（C17385） |
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| 中文摘要: |
| 【目的】比较黄龙病菌感染对长春花叶、茎、根 3 个组织中超氧化物岐化酶（SOD）和过氧化物酶（POD）
活性的影响。【方法】采用分光光度计法测定黄龙病菌感染后长春花叶、茎、根 3 个组织中 SOD 和 POD 的活性。
【结果】染病长春花中 SOD 活性随感染时间的增加，逐渐下降，活性最低时，叶、茎和根 SOD 活性分别只有健
康对照的 61%、55% 和 47%。3 个组织间 SOD 活性总体表现为叶 > 茎 > 根。染病长春花中 POD 活性随感染时间
的增加呈现先上升后下降的趋势，叶、茎和根 POD 活性最高出现在嫁接后 30、35、35 d，活性分别为健康对照
的 205%、254% 和 194%，根中 POD 活性总体较叶、茎部高。【结论】黄龙病菌感染对长春花各组织中 SOD 和
POD 酶的活性均有较大影响，SOD 和 POD 活性的增加对长春花抵抗 CaLas 侵染起重要作用。 |
| 英文摘要: |
| ：【Objective】The objective is to compare the effects of Candidatus Liberibacter asiaticus(CaLas)
infection on the activity of superoxide dismutase (SOD) and peroxidase (POD) in three organs, i.e. leaves, stems and roots of
Catharanthus roseus. 【Method】The activities of SODand POD in the leaves, stems and roots of C. roseus infected by CaLas
were determined by spectrophotometer method.【Result】The activity of SOD in the affected C. roseus decreased gradually
with the increase of infection duration. When the activity was the lowest, the activity of SOD in the leaves, stems and roots
was only 61%, 55% and 47% respectively of that of the healthy control group. Among these three organs, the leaves had the
highest SOD activity while the roots had the lowest. The activity of POD in the affected C. roseus increased first and then
decreased with the increase of infection duration. The activity of POD in leaves, stems and roots reached the highest 30, 35
and 35 days after grafting, and was 205%, 254% and 194% respectively of that of the healthy control group. The activity of
POD in roots was much higher than that in leaves and stems【Conclusion】The infection of CaLas has a great effect on the
activity of SOD and POD in the organs of C. roseus. The increase of SOD and POD activities plays an important role in the
resistance of C. roseus to CaLas infection. |
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