| 余凤玉，宋薇薇，韩 轩.春羽炭疽病病原鉴定及生物学特性研究[J].广东农业科学,2019,46(10):76-81 |
|
查看全文
HTML
春羽炭疽病病原鉴定及生物学特性研究 |
| Research on Identification and Biological Characteristicsof the Pathogen of Philodendron bipinnatifidum |
| |
| DOI：10.16768/j.issn.1004-874X.2019.10.012 |
| 中文关键词: 春羽 炭疽病 胶孢炭疽菌 生物学特性 |
| 英文关键词: Philodendron bipinnatifi dum anthracnose Colletotrichum gloeosporioides biological characteristics |
| 基金项目:2018 年海南省科学普及专项（SQ2017KPJD0004） |
|
| 摘要点击次数: 1376 |
| 全文下载次数: 614 |
| 中文摘要: |
| 【目的】在海南省文昌市椰子大观园露地种植的春羽（Philodendron bipinnatifi dum Schott ex
Endl.）上发现一种叶斑病，明确了该病害的病原物和部分生物学特性。【方法】通过分离培养和接种试验，经
传统形态学及分子生物学方法鉴定了致病菌，采用十字交叉法和血球计数板法测定该病原菌的生物学特性。【结
果】该病原菌鉴定为胶孢炭疽菌（Colletotrichum gloeosporioides），菌丝初期为白色，后转为青灰色，产生红
色孢子堆。分生孢子椭圆形，大小为 6.14~14.69 μm × 2.96~6.49 μm。菌丝生长最适温度为 25 ℃，最适 pH
值为 8~9；产孢最适温度为 30 ℃，最适 pH 为 9。此菌能利用多种碳源，但利用能力有差异，最适菌丝生长的
碳源为蔗糖；最适氮源为硝酸钠，硝酸铵和硫酸铵作为氮源培养基明显不利于菌丝生长。【结论】首次对春羽
炭疽病病原进行分离鉴定，该病原菌最适生长温度为 25 ℃，pH 为 9，最适碳氮源分别为蔗糖和硝酸钠。 |
| 英文摘要: |
| 【Objective】A new leaf spot disease was found from Philodendron bipinnatifi dum in Wenchang, Hainan
Province and its pathogen and biological characteristics were identified. 【Method】Isolation culture and inoculation
test were carried out, and traditional morphology and molecular methods was adopted identify the pathogen. The crossing
method and the blood cell count plate method were used to determined its biological characters. 【Result】The pathogen
was identified as by Colletotrichum gloeosporioides. The colony of the pathogen was white at the initial stage and then
changed to green-grey with red spore piles. The conidia were ellipse with the mean size of 6.14~14.69 μm × 2.96~6.49
μm. The optimum temperature for the colony growth was 25℃ . The optimum pH value was 9. In the tested carbon sources,
the pathogen could make use of many kinds of carbon sources, but the effect was different. The optimum carbon source and
nitrogen source were sucrose and sodium nitrate, respectively. Obviously, ammonium nitrate and ammonium sulfate(as nitrogen
sources) were not conducive for the mycelium growth. 【Conclusion】The pathogen of anthracnose in P. bipinnatifi dum was
isolated and identified for the first time. The optimum growth temperature and pH for the growth of the pathogen were 25℃
and 9, respectively. The optimum carbon and nitrogen sources were sucrose and sodium nitrate. |
|
查看/发表评论 下载PDF阅读器 |
