| 白永亮 1，2，凌志洲 1
，陈甜妹 1
，曾 荣 1
，夏 雨 3，吴俏槿 3，刘霭莎 3.青稞种子萌动过程中抗氧化活性的变化[J].广东农业科学,2019,46(11):119-126 |
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青稞种子萌动过程中抗氧化活性的变化 |
| Changes in Antioxidant Activity of HighlandBarley Seeds During Germination |
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| DOI：10.16768/j.issn.1004-874X.2019.11.017 |
| 中文关键词: 青稞 浸麦 萌芽 抗氧化活性 γ- 氨基丁酸（GABA） |
| 英文关键词: highland barley soaking germinating antioxidant activity γ-aminobutyric acid (GABA) |
| 基金项目:广东省中山市科技计划项目（2017A1032）；广东省农村科技特派员选派对接项目（bgk43207）；国家级大学生创新创业训练计划项目（201811847028） |
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| 中文摘要: |
| 【目的】探究青稞种子萌动过程中青稞芽水提物的体外抗氧化活性。【方法】以羟自由基的清除率
和金属离子还原能力为评价指标，研究了青稞种子浸麦和萌芽过程中体外抗氧化活性和 4 种功能性成分〔黄酮、
总酚、β- 葡聚糖、γ- 氨基丁酸（GABA）〕含量的变化规律，进行相关性分析，并对比了青稞芽、麦芽、谷芽
和稻芽的抗氧化活性。【结果】随着浸麦时间和萌芽时间的延长，青稞芽的抗氧化活性均呈先升后降的趋势，在
浸麦处理 6 h 达到最高点（羟自由基的清除率为 64.88%，金属离子还原能力为 0.910），萌芽处理 8 h 达点最高点（羟
自由基的清除率为 71.23%，金属离子还原能力为 1.337）。相关分析结果表明，青稞种子浸麦过程中 GABA 含量
与抗氧化活性显著相关；青稞芽、麦芽、谷芽和稻芽 4 种谷物芽粉的抗氧化活性均随样品浓度升高而增强，相同
浓度下，麦芽羟自由基的清除率最大，青稞芽金属离子还原能力最小。【结论】种子萌动处理有利于提高青稞的
抗氧化活性，可作为一种有效的改性方法用于青稞功能性产品的开发，促进青稞资源的深加工利用。 |
| 英文摘要: |
| 【Objective】In vitro antioxidant activity of water extract from highland barley sprout during germination
was investigated.【Method】Taking clearance rate of hydroxyl free radical and reduction capacity of metal ions as the
evaluation indicators, the change rules of in vitro antioxidant activity of highland barley and contents of four functional
ingredients〔flavonoids, total phenols, β-glucan, γ-aminobutyric acid (GABA)〕in the process of soaking and
germination were studied, and correlation analysis were conducted. And the antioxidant activity of highland barley sprout,
malt, millet sprout and rice sprout was compared.【Result】The results showed that, with the extension of soaking time and
germination time, the antioxidant activity of highland barley sprout showed the trend of rising first and then falling, and it
reached the peak under soaking treatment for 6 h (clearance rate of hydroxyl free radical was 64.88% and reduction capacityof metal ions was 0.910), while the value reached the peak under germination treatment for 8 h (clearance rate of hydroxyl free
radical was 71.23% and reduction capacity of metal ions was 1.337). The correlation analysis showed that there was a significant
correlation between the GABA content and the antioxidant activity during highland barley soaking. The antioxidant activity of
the four kinds of grain(highland barley sprout, malt, millet sprout and rice sprout) bud powders increased with the increase of
sample concentration. At the same concentration, the hydroxyl radical scavenging rate of malt was the highest, and the metal ion
reduction capacity of highland barley sprout was the lowest. 【Conclusion】 Germination treatment can improve the antioxidant
activity of highland barley. It can be used as an effective modification method to develop functional products of highland barley
and promote the deep processing and utilization of highland barley resources. |
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