| 邓 翔，李 祥，唐 奔，秦 祁，杨从发.响应面优化微波辅助提取桑叶蛋白工艺研究[J].广东农业科学,2021,48(5):141-148 |
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响应面优化微波辅助提取桑叶蛋白工艺研究 |
| Response Surface Optimization of Microwave-assisted Extraction of Mulberry Leaf Protein |
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| DOI：10.16768/j.issn.1004-874X.2021.05.018 |
| 中文关键词: 桑叶 叶蛋白 微波 响应面 考马斯亮蓝法 |
| 英文关键词: mulberry leaf leaf protein microwave response surface Bradford |
| 基金项目:江苏省海洋资源开发研究院开放课题（HKK201617） |
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| 中文摘要: |
| 【目的】在碱法提取桑叶蛋白的基础上，利用微波辅助提取方法探索不同条件下从桑叶中提取叶蛋白的效果。【方法】以桑叶蛋白提取率作为考察指标，在单因素试验基础上利用响应面试验对提取桑叶蛋白的工艺参数进行优化。【结果】随着浸提液 pH 值逐渐上升，桑叶蛋白提取率随之上升，在浸提液 pH=13 时，桑叶蛋白提取率达到峰值；随着料液比减小，桑叶蛋白提取率明显上升，当料液比达到 1 ︰ 100 g/mL 时，桑叶蛋白提取率达到峰值；随着微波提取时间延长，桑叶蛋白提取率呈现先上升后下降的趋势，在提取 80 s 时达到峰值；桑叶蛋白提取率随微波功率的逐渐增加显著上升，在功率为 500 W 时达到峰值。【结论】响应面法优化微波辅助碱法提取桑叶蛋白的最佳工艺参数为：pH 11.4，料液比 1 ︰ 102 g/mL，微波功率 500 W，微波提取时间 82 s，该条件下桑叶蛋白的提取率为 35.84%，与模型预测值相近。此方法大幅减少桑叶蛋白提取时间，为桑叶蛋白进一步研究利用提供基础。 |
| 英文摘要: |
| 【Objective】On the basis of alkaline extraction of mulberry leaf protein, microwave-assisted extraction method was used to explore the effects of leaf protein extraction from mulberry leaves under different conditions.【Method】The extraction yield of mulberry leaf protein was taken as the index. Based on the single factor test, the process parameters of extracting mulberry leaf protein were optimized by response surface test.【Result】With the gradual increase of the pH value of the extraction solution, the extraction yield of mulberry leaf protein increased. When the pH value of the extraction solution was 13, the extraction yield of mulberry leaf protein reached the peak. With the decrease of the solidliquid ratio, the extraction yield of mulberry leaf protein increased obviously, and reached the peak when the solid-liquid ratio reached 1 ∶ 100 g/mL. With the extension of microwave extraction time, the extraction yield of mulberry leaf protein increased first and then decreased, and reached the peak at 80 s. The extraction yield of mulberry leaf protein increased significantly with the increase of microwave power and reached the peak at 500 W.【Conclusion】The optimal extraction
parameters of mulberry leaf protein by microwave-assisted alkaline extraction were as follows: pH 11.4, solid-liquid ratio 1 ∶ 102 g/mL, microwave power 500 W, and microwave extraction time 82 s. Under these conditions, the extraction yield of mulberry leaf protein was 35.84%, which was close to the predicted value of the model. This method greatly reduced the extraction time of mulberry leaf protein and provided a basis for further study and utilization of mulberry leaf protein. |
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