| 田耀加，王秋燕，吴 蓓，叶伟忠，陈红弟.鲜食玉米抗南方锈病种质资源鉴定筛选[J].广东农业科学,2021,48(7):111-117 |
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鲜食玉米抗南方锈病种质资源鉴定筛选 |
| Identification and Selection of Fresh Corn Germplasm Resources with Resistance to Southern Corn Rust |
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| DOI：10.16768/j.issn.1004-874X.2021.07.014 |
| 中文关键词: 鲜食玉米 南方锈病 种质资源 抗性鉴定 遗传分析 |
| 英文关键词: fresh corn southern corn rust germplasm resource resistance identification genetic analysis |
| 基金项目:广州市珠江科技新星（201806010108）；广州市 2021 年农业财政专项资金（20101264） |
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| 中文摘要: |
| 【目的】研究不同鲜食玉米种质资源对南方锈病的抗性表现，为抗病品种选育提供材料及理论依据。
【方法】结合田间自然感病与人工接种感病目测鉴定调查，开展自交系对南方锈病的抗性分析。【结果】不同
鲜食玉米自交系对南方锈病的抗性存在显著差异，在 710 份材料田间自然感病鉴定中，1.11% 表现高抗水平，8.34%
表现为抗病水平；中抗和感病水平的材料最多，占 77.75%，其余材料表现为高感水平。结合人工接种鉴定，筛
选得到 5 份高抗南方锈病的自交系，包括 3 份糯玉米材料、1 份甜玉米材料和 1 份甜加糯玉米材料。对抗病自交
系 09N1-1-1 初步遗传分析显示，其后代 F2 和 BC1F1 群体抗感分离结果符合 3 ∶ 1（χ2 值 2.473，P=0.116）和 1 ∶ 1
（χ2 值 0.084，P=0.772）分离比例，表明自交系 09N1-1-1 对南方锈病的抗性可能由显性单基因控制。【结论】
研究表明，现有鲜食玉米存在高抗南方锈病的资源，筛选利用抗病种质对当前品种选育具有潜在的应用价值。 |
| 英文摘要: |
| 【Objective】The resistance of fresh corn germplasm resources to southern corn rust was explored with
a view to provide materials and theoretical references for resistant varieties.【Method】Investigation was conducted to
analyze the resistance of different inbred lines to southern corn rust with the method of visual observation under field natural
conditions and artificial inoculation.【Result】There were significant differences between different inbred lines of fresh
corn. Under field natural conditions, only 1.11% of 710 tested inbred lines showed highly resistant level, and 8.34% of which
showed resistant level. About 77.75% of inbred lines showed middle resistant and susceptible levels, while the rest showed
highly susceptible level. After artificial inoculation, 5 inbred lines of fresh corn that highly resistant to the southern corn rust
were screened out, including three of waxy corn, one of sweet corn and one of waxy-sweet corn. Preliminary genetic analysis
of the resistant inbred line 09N1-1-1 showed that the separation ratios of F2
and BC1F1
populations were 3 ∶ 1（χ2
=2.473，
P=0.116）and 1 ∶ 1（χ2
=0.084，P=0.772）respectively, revealing the resistance of inbred line 09N1-1-1 was probably
controlled by a single dominant gene.【Conclusion】The study demonstrated that the current fresh corn resources have
high resistance to southern corn rust. Therefore, the selection and utilization of disease resistant germplasm has potential
application in variety breeding currently. |
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