| 刘计涛，王梦诗，索海翠，罗焕明，王 丽，李成晨，单建伟，廖永珊，李小波.茉莉酸对马铃薯苗期低温胁迫耐性的影响[J].广东农业科学,2021,48(12):129-137 |
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茉莉酸对马铃薯苗期低温胁迫耐性的影响 |
| Effects of Jasmonic Acid on Cold Stress Tolerance of Potato at Seedling Stage |
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| DOI：10.16768/j.issn.1004-874X.2021.12.015 |
| 中文关键词: 马铃薯 茉莉酸 低温胁迫 活性氧 CBF 基因 |
| 英文关键词: potato, jasmonic acid, cold stress, reactive oxygen species, CBF gene |
| 基金项目:广东省农业科学院创新基金（202109）；广东省自然科学基金（2021A1515010778）；广州市科技计划项目（202102020845）；广东省现代农业产业技术体系创新团队项目（2021KJ111） |
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| 中文摘要: |
| 【目的】研究不同茉莉酸（JA）施用浓度对马铃薯叶片活性氧（ROS）含量及其清除酶活性以及
抗寒关键基因 CBF 表达的影响，初步确定提升马铃薯抗寒性的最适施用浓度，为马铃薯产业的发展提供一定的
理论基础。【方法】以马铃薯品种费乌瑞它为研究对象，采用叶面喷施 JA 的方法，探究正常和低温胁迫条件下，
植株叶片的活性氧含量，活性氧清除酶过氧化氢酶（CAT）、过氧化物酶（POD）和超氧化物歧化酶 SOD 活性，
以及抗寒关键基因 CBF 的表达。【结果】随着 JA 浓度的增加，马铃薯苗期叶片的活性氧含量逐渐降低，低温
胁迫后活性氧含量能维持在较低水平，适宜浓度范围为 100~400 μmol/L，以 200 μmol/L JA 处理效果最好，ROS
含量降低 47.6%。JA 能够提升活性氧清除酶 CAT 和 POD 活性，且随着 JA 浓度增加效果越显著，低温胁迫后
200 μmol/L JA 处理分别显著提升 1.30、2.53 倍。正常条件下，JA 处理抑制 SOD 活性最低达 70.7%；低温胁迫条
件下，JA 又可能通过其他途径提升 SOD 活性，最高达 2.11 倍。外源 JA 能诱导 CBF1-3 基因的表达，而低温胁
迫能促进内源 JA 的合成，诱导 CBF1-3 基因的表达，提升马铃薯的抗寒性。【结论】马铃薯苗期的抗寒性与外
源施加 JA 的浓度具有明显的相关性，适宜的 JA 浓度能够促进抗寒关键基因 CBF1-3 的表达，显著提升 CAT、
POD、SOD 的活性，抑制低温胁胁迫诱导的活性氧生成，提高马铃薯抗寒性。适宜施用 JA 浓度为 100~400 μmol/L，
以 200 μmol/L 效果最佳。 |
| 英文摘要: |
| 【Objective】The research was carried out to explore the effects of different jasmonic acid（JA）
concentrations on the content of reactive oxygen species（ROS）and its scavenging enzyme activity as well as the
expression of key cold resistance gene CBF of potato, and to identify the optimal application concentration of JA, which
contributed to enhance cold tolerance of potato, with a view to providing a theoretical basis for the development of potato
industry.【Method】Taking the potato variety Favorita as the research object, the method of spraying JA on potato leaves
was used to explore the content of ROS, the activities of catalase（CAT）, peroxidase（POD）and superoxide dismutase SOD, and the expression of CBF genes of potato leaves under the normal and low temperature conditions.【Result】As the
increase of JA concentration, the content of ROS of potato leaves at seedling stage decreased gradually, and the content of
ROS could be maintained at a low level upon cold stress. The appropriate concentration range was 100 μmol/L to 400 μmol/L,
among which 200 μmol/L had the most significant effect, and the ROS content was reduced by 47.6% compared with control.
JA could enhance the activities of ROS scavenging enzymes - CAT and POD, and the effect became more significant with the
increase of JA concentration. After cold stress, 200 μmol/L JA treatment could significantly increase the activities of CAT
and POD by 1.30 and 2.53 times, respectively. However, under the normal condition, JA treatment inhibited SOD activity to a
minimum of 70.7%, while JA may increase SOD activity to a maximum of 2.11 times through other ways under the condition
of cold stress. Exogenous JA could induce the expression of StCBF1-3, and cold stress could promote the synthesis of
endogenous JA which might induce the expression of StCBF1-3 and improve the cold tolerance of potato. 【Conclusion】The
cold tolerance of potato is significantly correlated with the concentration of exogenous JA. The appropriate concentration can
promote the expression of key gene StCBF1-3 for cold tolerance, significantly enhance the activities of CAT, POD and SOD,
inhibit the generation of ROS induced by cold stress, and improve the cold tolerance of potato. The optimal concentration of
JA is 100 μmol/L to 400 μmol/L, and 200 μmol/L has the best effect. |
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