| 毛美琴，蓝桢宇，李 进，郭锡燚，黄凤萍，廖元龙，蔡小辉.鱼类细胞培养历史、应用及培养条件研究概述[J].广东农业科学,2022,49(3):124-134 |
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鱼类细胞培养历史、应用及培养条件研究概述 |
| An Overview of Research on Culture History,Application and Culture Conditions of Fish Cell |
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| DOI：10.16768/j.issn.1004-874X.2022.03.014 |
| 中文关键词: 细胞培养技术 鱼类细胞 应用现状 发展方向 |
| 英文关键词: cell culture technique fish cells application status development direction |
| 基金项目:广西自然科学基金（2018GXNSFAA281208 和 2019GXNSFBA245062）；广西科技开发项目（桂科AB18221112）；广西北部湾海洋珍稀物种养护自治区重点实验室项目（2021ZB01） |
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| 中文摘要: |
| 细胞培养指的是经过酶、机械或化学的方法将组织离散成单个细胞，模拟动物体内环境进行培养
并促进其生长发育的方法。基于鱼类模型的潜在应用，开发了包括体外细胞培养在内的多种技术。20 世纪生物
技术兴起，体外细胞培养逐渐渗透至各学科应用，如免疫学、药理学、毒理学、遗传及组织再生和移植等。体
外培养细胞具有模仿宿主细胞遗传同质性的优点，实验过程中可一定程度上减少体内的反应变异性，且成本低、
重复性好、道德约束小。鱼类细胞培养技术发展于 20 世纪 60 年代，最早应用于病毒分离，细胞可从不同器官
组织中获得。虽然鱼类细胞培养技术研究晚于哺乳动物，但从长远来看对于鱼类细胞的深入研究对于理论研究
和实际应用都具有深远意义和广阔开发前景。简要讨论了鱼类细胞培养的历史进程、应用现状以及细胞培养技术，
为未来鱼类细胞培养技术研究提供参考。 |
| 英文摘要: |
| Cell culture refers to the method that tissues are separated into single cells by enzyme, mechanical or
chemical methods, and the growth and development of cells are simulated by the animal’s internal environment. Based on
the potential applications of the fish model, various tools and techniques, including in vitro cell culture, have been developed.
With the rise of biotechnology in the 20th century, in vitro cell culture has gradually been penetrated into various disciplines,
such as immunology, pharmacology, toxicology, genetics and tissue regeneration and transplantation. In vitro cultured cells
have the advantage of imitating the genetic homogeneity of host cells, and the reaction variability in vivo can be reduced to
a certain extent in the experimental process, with low cost, good repeatability, and less moral constraints. Fish cell culture
technology was developed in the 1960s and was first applied to virus isolation. Cells can be obtained from different organs
and tissues. Although the research of fish cell culture technology is later than that of mammals, in the long run, the indepth study of fish cells has far-reaching significance and broad development prospects for both theoretical research and
practical application. This review briefly discusses the history, application and technology of fish cell culture, which provides
reference for future research on fish cell culture technology. |
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