| 蒋智勇，楚品品，陈天宝，李春玲，蔡汝健.广东省猪繁殖与呼吸综合征病毒ORF5 基因遗传变异分析[J].广东农业科学,2022,49(4):97-105 |
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广东省猪繁殖与呼吸综合征病毒ORF5 基因遗传变异分析 |
| Genetic Variation Analysis of ORF5 Genes of Porcine Reproductive and Respiratory Syndrome Virus in Guangdong Province |
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| DOI：10.16768/j.issn.1004-874X.2022.04.012 |
| 中文关键词: 猪繁殖与呼吸综合征病毒（PRRSV） ORF5 基因 NADC30 毒株 NADC34 毒株 遗传变异 |
| 英文关键词: Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) ORF5 gene NADC30 strain NADC34 strain genetic variation |
| 基金项目:广东省动物疫病野外科学观测研究站项目（2021B1212050021）；广东省重点领域研发计划项目（2019B020217002）；广东省农业科学院院长基金（202044） |
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| 中文摘要: |
| 【目的】监测 2021 年广东地区猪繁殖与呼吸综合征病毒 (Porcine Reproductive and Respiratory
Syndrome Virus，PRRSV) 的分子流行动态，为 PRRSV 综合防控措施的制定提供参考。【方法】在广东省内不同
地区 PRRSV 检测为阳性的 37 个规模猪场采集 521 份患病猪组织样本进行 PRRSV 检测。对样本进行 ORF5 基因
测序，采用 DNAStar 分析 ORF5 核苷酸的同源性及其推导氨基酸的同源性，并用 Mega7.0 构建基于 ORF5 基因的
系统遗传进化树。【结果】从 37 个猪场采集的样品 PRRSV 阳性率为 24.4%，猪场阳性率为 45.9%。通过测序分
析获得 17 株来自不同猪场的 PRRSV ORF5 基因序列，遗传进化分析表明所有毒株均属于北美毒株（PRRSV 2），
其中有 5 株属于 Lineage 8( JXA1-like 和 CH-1a-like）谱系，9 株属于 Lineage 1( NADC30-like 和 NADC34-like）谱系，
3 株属于 Lineage 3（QYYZ-like）谱系，没有监测到 Lineage 5（VR2332-like）谱系的毒株。17 株 PRRSV 毒株的
ORF5 基因之间核苷酸序列同源性为 81.3%~99.5%，推导的氨基酸序列同源性为 80.0%~98.3%。【结论】2021 年
广东省 PRRSV 流行株以 Lineage 1（NADC30-like 和 NADC34-like）和 Lineage 8（JXA1-like 和 CH-1a-like）谱
系为主，占比分别为 52.94% 和 29.41%，Lineage 3（QYYZ-like）谱系降为次要流行株，占比为 17.65%。 |
| 英文摘要: |
| 【Objective】The molecular epidemiological dynamics of porcine reproductive and respiratory syndrome
virus (PRRSV) in Guangdong Province in 2021 were monitored in order to provide references for the formulation of comprehensive prevention and control measures for PRRSV.【Method】521 tissue samples of diseased pigs were selected from
37 large-scale farms that were tested positive as PRRSV in different regions of Guangdong Province for ORF5 gene sequencing,
then DNAStar was used to analyze the nucleotide homology and deduced amino acid homology of the ORF5 gene, and Mega
7.0 was used to construct ORF5-based phylogenetic tree.【Result】Positive rate of PRRSV was 24.4% in the collected
samples, and the positive rate of pig farms was 45.9%. The ORF5 gene sequences of 17 strains of PRRSV from different pig
farms were obtained by sequencing. Phylogenetic analysis showed that all the isolates belonged to the North America genotype.
Among them, 5 strains belonged to the Lineage 8 (JXA1-like and CH-1a-like) subgroup, 9 strains belonged to the Lineage
1 (NADC30-like and NADC34-like) subgroup, 3 strains belonged to the Lineage 3 (QYYZ-like) subgroup, and no strains
of the Lineage 5 (VR2332-like) were detected. The nucleotide sequence homology of ORF5 gene of 17 strains was 81.3%-
99.3%, and the deduced amino acid sequence homology was 80.0%-98.3%. 【Conclusion】The predominate strains of PRRS
in Guangdong Province in 2021 belonged to Lineage 1 (NADC30-like and NADC34-like) and Lineage 8 (JXA1-like and CH-
1a-like), accounting for 52.94% and 29.41%, respectively. Lineage 3 (QYYZ-like) was reduced to a secondary epidemic strain,
accounting for 17.65%. |
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