| 李望豪，周瑞鹏，许钰滢，赵琪琪，刘 娟，王荣波，叶文雨.一株海南菌草“绿洲一号”内生细菌的分离鉴定及其生物学特性研究[J].广东农业科学,2022,49(7):81-88 |
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一株海南菌草“绿洲一号”内生细菌的分离鉴定及其生物学特性研究 |
| Isolation, Identification and Biological Characteristics of An Endophytic Bacterium from Juncao“Oasis No.1”in Hainan |
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| DOI：10.16768/j.issn.1004-874X.2022.07.011 |
| 中文关键词: 菌草 内生细菌 生物学特性 拮抗活性 促生潜力 |
| 英文关键词: Juncao endophytic bacteria biological property antagonistic activity growth-promoting property |
| 基金项目:福建农林大学科技创新专项基金（KFA19137A）；福建省作物有害生物监测与治理重点实验室开放课题（MIMCP-202002）；福建省科技重大专项（2021NZ029009）；福建省引导性科技计划项目（2022N0010） |
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| 中文摘要: |
| 【目的】探讨 1 株从海南菌草“绿洲一号”叶片中分离鉴定的内生细菌 HLY3-2 的促生、拮抗
病原真菌等特性，为植物病原真菌的生物防治及植物促生机理研究提供基础理论。【方法】以海南菌草“绿洲
一号”叶片为材料，运用稀释涂布平板法分离内生细菌 HLY3-2。通过形态学鉴定、16SrDNA 序列测定及同源性
分析，构建系统发育树确定该菌属种。采用平板对峙法并利用各种生理生化培养基、促生性能培养基研究其生
物学特性。【结果】通过内生细菌形态学、生理生化特性和 16SrDNA 序列测定，HLY3-2 被鉴定为苏云金芽孢
杆菌（Bacillus thuringiensis）。拮抗病原真菌筛选试验结果表明，内生细菌 HLY3-2 对稻瘟病菌（Magnaporthe
oryzae, Guy11）、香蕉枯萎病菌 4 号（Foctropical race4, Foc4）均有一定的抑菌作用，抑菌率分别为 18.45% 和
30.65%。生理生化特性分析结果表明，该内生细菌不具有利用柠檬酸盐、纤维素与淀粉的能力；在生长过程
中不产生酯类物质，也无法将明胶水解，但其在生长过程中具有产生蛋白酶和过氧化氢酶的能力。促生性能分
析结果表明，该内生细菌具溶解无机磷和有机磷、降解硅酸盐的能力，不具有产铁载体潜力，但具有很强的产
IAA 能力。【结论】内生细菌 HLY3-2 在促进植物生长和防治病害中有一定作用。 |
| 英文摘要: |
| 【Objective】The study aimed to explore the growth-promoting properties and antagonism of pathogenic
fungi of an endophytic bacterium HLY3-2 isolated and identified from the leaves of Hainan Juncao“Oasis No.1”, with a
view to providing basic theory for the biological control of plant pathogenic fungi and the study of plant probiotic mechanism.
【Method】The endophytic bacterium HLY3-2 was isolated from the leaves of Hainan Juncao “Oasis No.1” by using the
dilution plating method. Through morphological identification,16SrDNA sequencing and homology analysis, a phylogenetic
tree was constructed to identify the genus and species of this strain. The biological characteristics were explored by using the
plate antagonism method and various physiology and biochemistry culture medium and growth-promoting property culture
medium.【Result】HLY3-2 was identified as a strain of Bacillus thuringiensis through the endophytic bacterium morphology,
physiology and biochemistry characteristics and 16SrDNA sequencing. The results of antagonism of pathogenic fungi showed
that the strain HLY3-2 had some inhibition effect on Magnaporthe oryzae and Foctropical race4 with the bacteriostasis rates
of 18.45% and 30.65% respectively. Then, according to results of the analysis on physiology and biochemistry characteristics,
the strain did not have the ability of utilizing citrate, cellulose and starch. And it could not produce ester substances and
hydrolyze gelatin during growth, but it had the ability of producing proteases and catalase during its growth and metabolism
process. The results of growth-promoting property analysis showed that the strain had the ability to dissolve inorganic
phosphorus and organophosphorus and degrade silicates, and could not produce iron carriers, but had strong IAA-producing
ability.【Conclusion】The experiment results show that the endophytic bacterium HLY3-2 has a role in promoting plant growth and
controlling disease. |
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