| 殷志斌 1
，陈旭峰 1
，江 彪 2
，晏石娟 1.脂质分析技术及其在植物种子脂质
代谢调控研究中的应用进展[J].广东农业科学,2022,49(11):75-85 |
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脂质分析技术及其在植物种子脂质
代谢调控研究中的应用进展 |
| Progress in Lipid Analysis Techniques and Their Applicationsto Studies of Lipid Metabolic Regulation in Plant Seeds |
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| DOI：10.16768/j.issn.1004-874X.2022.11.009 |
| 中文关键词: 脂质组学 质谱成像 空间分辨 可视化检测 脂质代谢调控 植物种子 |
| 英文关键词: lipidomics mass spectrometry imaging spatially resolved visual detection lipid metabolic regulation plant seeds |
| 基金项目:广东省农业科学院协同创新中心项目（XTXM202203）；广东省农业科学院高水平农科院建设-科技
创新战略专项（R2020PY-JX019；R2021YJ-QG004）；广州市“青年科技人才托举工程”项目（QT20220101031）；
广东省重点领域研发计划项目（2020B0202090005） |
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| 中文摘要: |
| 脂质是一类具有独特生理功能活性的化合物，参与调节多种植物应答非生物胁迫过程，对维持植
物组织中生理动态平衡至关重要。脂质组学（Lipidomics）自 2003 年被提出后，已迅速发展成为对脂质整体系
统分析的一门新兴学科，为传统代谢组学注入新的技术支撑，有助于阐明脂类物质在植物中的代谢调控机制。
同时，质谱成像技术因其具有无标记、非特异性、高灵敏度、多物质同时分析等优势，被广泛应用到植物组织
中各类脂质分子的空间分布研究。介绍了脂质组学和质谱成像技术的研究现状，重点综述脂质分析技术在植物
种子脂质代谢调控研究中的最新进展，特别是新兴质谱成像技术在植物种子中脂类物质的成像应用。脂质组学
和质谱成像技术作为目前多组学技术的重要补充，将为植物代谢途径和调控机制的深入探究提供新的契机。亚
微米级高空间分辨率质谱成像技术的不断发展，将进一步推动植物在空间分辨水平的脂质代谢调控网络的深入
解析和前沿应用研究。 |
| 英文摘要: |
| Lipids are a class of compounds with unique physiological functions and activities. They participate in the
regulation of a variety of plant responses to abiotic stress, which are essential for maintaining physiological homeostasis in
plants. Since its proposal in 2003, lipidomics has rapidly developed as an emerging discipline that provides novel technical
support for traditional metabolomics. Lipidomics can aid in clarifying the mechanisms of lipid metabolic regulation in plants. Moreover, the advantages of being label-free, non-specific, highly sensitive an d allowing for simultaneous elemental
and molecular analysis mass spectrometry imaging (MSI) has been widely used to decipher the spatial distribution of various
lipid molecules in plant tissues. Here, we introduce the current research status of lipidomics and MSI technologies, and
comprehensively review recent progress in applying lipid analysis techniques to study lipid metabolic regulation in plant seeds,
especially the application of MSI techniques for mapping lipid species in plants. Lipidomics and MSI are important complements
to commonly used multi-omics technologies, and they will provide new opportunities for deeper exploration of plant metabolic
pathways and regulatory mechanisms. The continuous development of high-spatial-resolution MSI technologies is expected to
further promote the deep analysis and prospecting applications of lipid metabolism network regulation at the spatially resolved
level in plants. |
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