| 梅文宇 1,2，方燕芬 1，宫 超 1，麦培婷 1，孙保娟 1，李植良 1，李 涛 1，孙光闻 2.茄子 TCP 转录因子的鉴定及胁迫处理下的表达分析[J].广东农业科学,2022,49(12):20-33 |
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茄子 TCP 转录因子的鉴定及胁迫处理下的表达分析 |
| Genome-Wide Identification and Expression Analysis in Oxidative Stress of TCP Transcription Factor Family in Eggplant (Solanum melongena L. ) |
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| DOI：10.16768/j.issn.1004-874X.2022.12.003 |
| 中文关键词: 茄子 TCP 基因家族 逆境胁迫 激素处理 表达分析 |
| 英文关键词: eggplant TCP gene family adversity tress hormone treatment expression analysis |
| 基金项目:广东省自然科学基金（2021A1515012490，2020A1515011330）；广东省现代农业产业技术体系（2021KJ106，2021KJ110）；广东省农业科学院项目（R2020PY-JX003，R2021YJ-YB3019，KTP20210064） |
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| 中文摘要: |
| 【目的】鉴定茄子 TCP 基因并对茄子 TCP 转录因子家族成员分析。确定其在胁迫、激素处理下的表达情况，为深入研究茄子 TCP 基因的功能奠定基础。【方法】从茄子基因组鉴定 SmTCP 基因家族成员，利用生物信息学方法对茄子 TCP 转录因子家族成员的理化性质、分布情况进行分析。以高温（42 ℃）、低温（4 ℃）、盐胁迫（200 μmol/L NaCl 溶液）、重金属（100 μmol/L CdCl2 溶液）、青枯菌、水杨酸及脱落酸处理茄子幼苗，利用 qRT-PCR 技术分析茄子 TCP 基因在 7 种处理及不同器官的表达情况。【结果】共鉴定到 32 个茄子 TCP 成员，并进行生物信息学分析。qRT-PCR 分析结果显示，茄子 SmTCP 基因受生物及环境胁迫响应上调，激素处理中，ABA 处理后茄子 TCP 整体出现上调，SA 处理后茄子 SmTCP 整体出现下调，茄子 TCP 在叶跟果中的的含量较高。【结论】茄子 TCP 基因受胁迫响应表达，SmTCP 成员响应胁迫表达情况及各器官中的表达量不同，Class Ⅰ类TCP 大多成员在热胁迫下存在明显上调响应。推测茄子 TCP 基因可能参与抵御逆境胁迫。 |
| 英文摘要: |
| 【Objective】The study was carried out to identify the SmTCP gene and analyze the members of SmTCP transcription factor family in eggplant. The determination of its expression under stress and hormone treatment laid a foundation for further study of the function of SmTCP gene in eggplant.【Method】The members of SmTCP gene family were identified from the eggplant genome. The physicochemical properties and distribution of members of SmTCP transcription factor family in eggplant were analyzed by bioinformatics methods. Eggplant seedlings were treated with high temperature (42 ℃ ), low temperature (4℃ ), salt stress (200 μmol/L NaCl solution), heavy metal (100 μmol/L CdCl2 solution), Pseudomonas solanacearum, salicylic acid and abscisic acid. The expression of SmTCP gene in eggplant under seven treatments was analyzed by qRT-PCR.【Result】A total of 32 SmTCP members were identified and analyzed by bioinformatics method. The results of qRT-PCR analysis showed that the SmTCP gene of eggplant was up-regulated in response to biological and environmental stress. Under hormone treatment, the SmTCP of eggplant was up-regulated generally after ABA treatment, and the SmTCP of eggplant was down-regulated generally after SA treatment. The contents of SmTCP in leaves and fruits were higher. 【Conclusion】It was found that the SmTCP gene of eggplant express in response to stress. The expression of different SmTCP members in response to stress and the expression levels in various organs are different. Most of the Class I TCP members of eggplant are significantly up-regulated under heat stress. Therefore, it is speculated that the SmTCP gene of eggplant may be involved in resisting adversity stress. |
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