| 张文武 1，邱峰芳 1，许继国 2.白耳黄鸡三叉冠性状分析[J].广东农业科学,2022,49(12):118-123 |
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白耳黄鸡三叉冠性状分析 |
| Analysis of the Trigeminal Comb Traits in White-ear Yellow Chicken |
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| DOI：10.16768/j.issn.1004-874X.2022.12.013 |
| 中文关键词: 全基因组关联分析 单核苷酸多态性 常染色体遗传 种蛋受精率 鸡冠型 |
| 英文关键词: genome-wide association analysis single nucleotide polymorphisms autosomal inheritance fertility of breeding egg comb type of chicken |
| 基金项目:国家自然科学基金（32160784）；广东省农业动物基因组学与分子育种重点实验室开放课题；江西省自然科学基金（20171BAB204013） |
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| 中文摘要: |
| 【目的】对白耳黄鸡三叉冠性状进行分析，以期寻找与三叉冠性状相关的候选单核苷酸多态性（Single Nucleotide Polymorphisms, SNP）位点。【方法】60 只白耳黄鸡用于杂交试验，120 只白耳黄鸡用于受精率试验。对 82 只白耳黄鸡采集血样抽提 DNA，用 Illumina 鸡 60 k 芯片进行基因型分型，利用 PLINK 1.90 对分型结果进行质控后，采用 GEMMA 软件对 SNP 与性状进行全基因组关联分析（Genome-wide Association Analysis, GWAS），寻找与白耳黄鸡三叉冠性状显著相关的 SNP 位点。【结果】白耳黄鸡三叉冠呈现常染色体遗传，其种蛋受精率与单冠的白耳黄鸡差异不显著。由 82 只白耳黄鸡群体得到 55 023 个有效 SNP，群体内不存在明显的群体分层。与三叉冠相关的 SNP 位点有 10 个，分别位于第 1、2、3、4、5、12、14 号染色体上，邻近或座落于 SPRY2、NDFIP2、ITGA9、EMC2、TMEM17、EHBP1、SCAF8、TIAM2、HTT、STON2、PRKCD、RBBP6、TNRC6A、MAPK8IP3 基因上。【结论】通过 GWAS 分析发现 10 个 SNP 位点可能与白耳黄鸡三叉冠性状相关。研究结果将为白耳黄鸡的育种提供候选分子标记，为地方鸡标记辅助选择提供新的思路。 |
| 英文摘要: |
| 【Objective】The study was carried out to find candidate single nucleotide polymorphisms (SNP) loci associated with the trigeminal comb traits in white-ear yellow chickens.【Method】Sixty white-ear yellow chickens were used for crossbreeding experiment and 120 white-ear yellow chickens were used for fertility rate experiment. The blood samples of eighty-two white-ear yellow chickens were collected for DNA extraction, and then genotype classification was conducted by using Illumina chicken 60 k microarrays. After using PLINK 1.90 for quality control of the genotyping results, GEMMA software was used to perform genome-wide association analysis (GWAS) on SNP and traits to find SNP loci significantly associated with trigeminal comb traits in white-ear yellow chickens.【Result】The trigeminal comb of white-ear yellow chicken showed autosomal inheritance and the egg fertility rate was not significantly different from that of the white-ear yellow chicken with single comb. 55 023 valid SNPs were obtained from the population of 82 white-ear yellow chickens and there was no significant population stratification within the population. Ten SNP loci associated with trigeminal
comb were located on chromosomes 1, 2, 3, 4, 5, 12 and 14 of white-ear yellow chickens, adjacent to or located on SPRY2, NDFIP2, ITGA9, EMC2, TMEM17, EHBP1, SCAF8, TIAM2, HTT, STON2, PRKCD, RBBP6 ,TNRC6A and MAPK8IP3 genes.【Conclusion】Based on the GWAS analysis, it is found that 10 SNPs loci may be associated with the trigeminal comb traits in white-ear yellow chickens. These results will provide candidate molecular markers for the breeding of white-ear yellow chickens and new ideas for marker-assisted selection of local chickens. |
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