李娟,王文丽,赵旭.生物肥料对当归生长及土壤酶活性、微生物多样性的影响[J].广东农业科学,2020,(6-7):- |
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生物肥料对当归生长及土壤酶活性、微生物多样性的影响 |
Effects of Biological Fertilizers on Angelica Growth and Soil Enzyme activity and Soil Microbial Diversity |
投稿时间:2020-04-29 修订日期:2020-07-16 |
DOI: |
中文关键词: 生物肥料 当归 促生 土壤酶 可培养微生物 微生物功能多样性 |
英文关键词: Biological fertilizer Angelica sinensts growth promotion soil enzyme Culturable microorganisms Microbial functional diversity |
基金项目:国家重点研发计划项目 “畜禽废弃物无害化处理与资源化利用新技术及新产品研发”子课题“固体废弃物生物降解技术及新型肥料产品研发” (2016YFD0501401)资助。 |
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中文摘要: |
【目的】研究当归专用生物肥料对当归的促生和增产效果、土壤根际微生物群落多样性和土壤酶活的影响,为当归生物肥料的推广应用提供依据。【方法】以岷归为试材,通过盆栽试验设置对照、载体、生物肥料3个处理,在当归收获时采集根际土壤,测定土壤中脲酶和磷酸酶活性,并采用boilog和平板稀释计数相结合的方法,考察当归根际土壤微生物数量和多样性。【结果】生物肥料对当归芦头直径、根的生长有显著的促进作用。与对照相比,芦头直经、根干重增幅分别为31.39%、100.5%;,与载体处理相比分别增加19.2%、56.1%。。与对照相比,生物肥料处理生物肥料可以增加根际土壤细菌、真菌、放线菌的数量,与对照相比,增幅分别达154%、21.6%、34.7%。,生物肥料能够增加土壤脲酶、酸性磷酸酶、碱性磷酸酶活性,增幅分别为46.9%、19.9%、43.0%。通过Biolog方法研究土壤微生物功能多样性发现,施用生物肥料后,AWCD值、Shannon-Wiener指数(H’)、McIntosh指数(U)显著提高,单一碳源D-木糖、葡萄糖-1-磷酸盐、L-苯基丙氨酸、甘氨酰-L-谷氨酸、D,L-a-甘油、I-赤藻糖醇、N-乙酰基-D-葡萄胺的利用率显著提高, L-丝氨酸、丙酮酸甲酯、腐胺、4-羟基苯甲酸、D-半乳糖醛酸、衣康酸、D-苹果酸7种碳源的利用率降低,使单一碳源的代谢发生变化。【结论】当归专用生物肥料能够显著促进当归根的生长,提高土壤微生物的数量、土壤脲酶、酸性磷酸酶、碱性磷酸酶活性。 |
英文摘要: |
【Objective】The effect of Angelica special biological fertilizer on growth promotion and yield increase of Angelica
and on the diversity of soil rhizosphere microbial community and soil enzyme activity was investigated, the results provide a theoretical basis for the popularization and application of Angelica biological fertilizer. 【Method】The test material was from Min county, Gansu province, China, the pot experiment was conducted with three treatments of control,carrier and bio-fertilizer, The rhizosphere soil was collected Angelica harvest, and the urease and phosphatase activities in the soil were determined, and the number and diversity of soil microorganisms in the in the rhizosphere of Angelica were investigated by Boilog method and plate dilution counting.【Result】Angelica biological fertilizer significantly promoted the diameter and the root growth of Angelica. Compared with the control, the diameter and dry weight of root growth of Angelica increased by 31.39% and 100.5%, respectively; Compared with the carrier treatment, increased by 19,.2% and 56.1%, respectively. Compared with the control, Angelica biological fertilizer significantly increased the number of rhizosphere soil bacteria,fungi and antinomycetes the increase by 154%, 21.6% and 34.7%, respectively. Compared with the control, the activity of soil urease,acid phosphatase and alkaline phosphatase with Angelica biological fertilizer increased by 46.9%, 19.9% and 43.0%, respectively. The functional diversity of soil microorganism was investigated by Biolog method, results showed that the AWCD value, the Shannon-Wiener index (H') and the McIntosh index (U) were increased significantly, and the utilization ratio rates of D-xylose,Glucose-1-phosphate,L-phenylalanine, Glycyl-L-glutamic acid, D,L-a-glycerol, I-erythritol, N-acetyl-D-glucosamine from single carbon source improved significantly,The utilization ratio of L-serine, Methyl pyruvate, Putrescine, 4-hydroxybenzoic acid, D-galacturonic acid, Itaconic acid, and D-malic acid from 7 carbon sources decreased.【Conclusion】Angelica biofertilizer can significantly promote the root growth of Angelica root, and increase the number quantity of soil microorganisms, and activity of soil urease, Acid phospatase and Alkaline phosphatase. |
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