| 陈博雯,肖玉菲,李军集,等.EuCAD基因小片段RNA干扰调控木质素合成[J].广东农业科学,2020,(6-7):- |
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EuCAD基因小片段RNA干扰调控木质素合成 |
| RNA interferenc triggered by small fragment of EuCAD gene regulates lignin synthesis |
| 投稿时间：2020-05-08 修订日期：2020-05-08 |
| DOI： |
| 中文关键词: 肉桂酰乙醇脱氢酶，RNA干扰片段，木质素合成，烟草转化 |
| 英文关键词: cinnamoyl alcohol dehydrogenase, RNA interference, lignin synthesis, tobacco transformation |
| 基金项目:广西林业科技项目“木质素合成调控高效RNA干扰片段筛选”(桂林科字[2016]第11号),广西科技基地和人才专项项目“转基因手段培育桉树纸浆材新品种研究创新创业青年人才培养示范”(桂科AD18281086)。 |
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| 中文摘要: |
| 【目的】为深入研究尾叶桉(Eucalyptus urophylla)肉桂酰乙醇脱氢酶(CAD)基因序列中的关键片段并利用其进行木质素合成调控。【方法】采用生物信息学工具对EuCAD基因序列分析,根据编码蛋白保守结构域的分布情况进行筛选,获得一段44bp关键序列用于构建RNA干扰载体并转化烟草,获得转基因植株。【结果】对转基因烟草植株检测发现,CAD基因表达水平降低至野生型的12.51%,木质素含量降低15.26%,木质部厚度较野生型降低10%以上,转基因植株茎部直径与生长发育与野生型无异。【结论】通过序列分析筛选出的EuCAD基因小片段,可通过RNA干扰显著抑制木质素合成,为尾叶桉木质素合成调控研究提供了技术参考。 |
| 英文摘要: |
| [Objective] In order to study the key sequence of cinnamoyl alcohol dehydrogenase (CAD) gene of Eucalyptus urophylla and use them to regulate lignin synthesis. [Methods] Sequence analysis of eucad gene was carried out by bioinformatics method. According to the distribution of conservative domain of coding protein, a 44 bp key sequence was obtained to construct RNA interference vector and transgenic tobacco plants were generated. [Results] In the transgenic plants, the results showed that the expression level of CAD gene decreased to 12.51% of the wild type, the content of lignin decreased by 15.26%, the thickness of xylem decreased by more than 10% compared with the wild type, and the stem diameter, growth and development of transgenic plants were the same as those of the wild type. [Conclusion] The small fragment of EuCAD gene screened by bioinformatics analysis can significantly regulate lignin synthesis by triggering RNA interference, which provides a technical reference for the study of the regulation of lignin synthesis in Eucalyptus urophylla. |
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