衡周,孙小晴,黄俊霖,等.茄子褐纹病菌的分离鉴定及产孢条件的优化 [J].广东农业科学,2020,(6-7):- |
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茄子褐纹病菌的分离鉴定及产孢条件的优化 |
Isolation and identification and Optimization of Spore Production Conditions of Phomopsis vexans Harter |
投稿时间:2020-05-18 修订日期:2020-06-02 |
DOI: |
中文关键词: 褐纹病菌 分子鉴定 液体培养 产孢 |
英文关键词: Phomopsis vexans MolecularSidentification liquid culture spore-forming conditions |
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中文摘要: |
【目的】由茄褐纹拟茎点霉(Phomopsis vexans Harter)引起的褐纹病是当前茄子产业的重要病害。孢子富集是深入研究褐纹病菌孢子萌发及其致病机理的基础,为进一步简化当前复杂的产孢过程,提高产孢效率。【方法】本研究采用组织分离法开展病原菌的分离纯化,利用形态学特征观察及rDNA-ITS序列分析对病原菌进行种类鉴定;使用液体培养的方式,针对茄子褐纹病菌的产孢条件进行了优化。【结果】分离获得的病原菌rDNA-ITS序列经NCBI和序列比对与茄子褐纹病菌遗传关系最近,同源性达99%;最高效的产孢方法为:在150rpm摇床中,使用马铃薯液体培养基,28℃培养一天后,24℃培养三天。【结论】本研究优化了褐纹病孢子产孢条件,为开展褐纹病菌孢子萌发及致病机理研究提供技术支持。 |
英文摘要: |
【Objective】 Phomopsis vexans Harter is the causative agent of Phomopsis blight of eggplant which is an important disease in the current eggplant industry . A sufficient amount of spores is the material basis for studying its spore germination and pathogenic mechanism. This research is aimed to further simplify the current complex sporulation process and improve the sporulation efficiency. 【Method】 In this study, the tissue separation method was used to isolate and purify the pathogen. The morphological characteristics observation and rDNA-ITS sequence analysis were used to identify the pathogen. The liquid culture method was used to optimize the sporulation conditions of Phomopsis vexans. 【Result】The rDNA-ITS sequence of Phomopsis vexans was obtained. The sequence has the closest genetic relationship with Phomopsis vexans through NCBI and sequence alignment, with a homology of 99%. The most efficient method of spore production is: in a 150 rpm shaker, using potato liquid medium, culture at 28 ° C for one day, and then culture at 24 ° C for three days. 【Conclusion】 This study optimized the spore-forming conditions of Phomopsis vexans, and provide technical support for research on spore germination and pathogenic mechanism of it. |
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