| 谭建彬,徐进,师沛琼,等.小菜蛾清道夫受体基因家族鉴定与生物信息学分析[J].广东农业科学,2023,(5-6):- |
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小菜蛾清道夫受体基因家族鉴定与生物信息学分析 |
| Genome-wide Identification and Bioinformatics Analysis of Scavenger Receptor Gene Family in Plutella xylostella |
| 投稿时间：2023-04-20 修订日期：2023-05-24 |
| DOI： |
| 中文关键词: 小菜蛾 清道夫受体 基因家族 CD36 RNAi 功能结构 |
| 英文关键词: Plutella xylostella scavenger receptor gene family CD36 RNAi functional structure |
| 基金项目:广东省基础与应用基础研究基金(2019A1515110745)；广东省科技专项资金(2020A06013)；广东海洋大学科研启动经费资助项目 (R19025,R19029)。 |
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| 摘要点击次数: 246 |
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| 中文摘要: |
| 【目的】小菜蛾(Plutella xylostella)对化学杀虫性抗药性逐年上升，使RNAi（RNA interference）农药广受关注。小菜蛾清道夫受体（Scavenger receptors, SCRs）在细胞免疫中表现出吞噬和清除病原体的能力，使其成为RNAi农药的备选靶标基因。为确定小菜蛾SCRs相关靶标，本研究鉴定分析了小菜蛾SCRs基因家族成员信息。【方法】基于全基因组数据，对小菜蛾清SCRs基因家族的理化性质，跨膜结构，系统发育进化，二三级结构预测，蛋白保守基序，磷酸化位点预测等内容进行分析。【结果】小菜蛾SCRs基因家族有12个CD36蛋白序列，具有相似的理化性质和功能结构，均含有多个亲水性和疏水性区域及2个跨膜螺旋区，二三级结构的主要特征为无规则卷曲，磷酸化位点中丝氨酸含量最高。小菜蛾，家蚕(Bombyx mori)，冈比亚按蚊(Anopheles gambiae)，黑腹果蝇(Drosophila melanogaster)，烟草天蛾(Manduca sexta)，赤拟谷盗(Tribolium castaneum共6个物种，包含的117条CD36蛋白序列在进化树上分成了5个分支，具有相似结构的基因聚为同一支，进化保守性强。CD36px4、CD36px5具有更复杂的三级结构，且motif19、motif20两个基序只存在于CD36px4，推测其在物种进化中发挥某些特异的功能，CD36px4磷酸化位点数量多达143个，可能与凋亡细胞的吞噬和清除功能表达有关。【结论】鉴定分析到12个小菜蛾SCRs蛋白是一种跨膜型蛋白，且含有丰富的磷酸化位点，预测其可参与机体活动，细胞内外物质传输、信号传递及能量代谢等活动，具有介导细胞凋亡、吞噬和清除的功能，可作为RNAi农药的备选靶标，为小菜蛾RNAi农药靶标的筛选提供重要依据。 |
| 英文摘要: |
| 【Objective】The Plutella xylostella SCRs have phagocytic and pathogen-clearing abilities in cellular immunity, rendering them a promising target for RNAi pesticides. This study explores alternative targets for such pesticides by investigating the SCRs gene family in Plutella xylostella.【Method】Based on the Plutella xylostella genome, we comprehensively analyzed the physicochemical properties, transmembrane structure, phylogenetic evolution, secondary and tertiary structure prediction, conserved protein motifs analysis and phosphorylation site prediction of SCRs gene family. 【Result】The SCRs gene family of Plutella xylostella has 12 CD36 protein sequences with similar physicochemical properties and functional structures, all of which contain multiple hydrophilic and hydrophobic regions as well as two transmembrane helical regions. Their secondary and tertiary structures feature irregular coiling with the highest serine content in the phosphorylation site. A total of six species, including Plutella xylostella, Bombyx mori, Anopheles gambiae, Drosophila melanogaster, Manduca sexta and Tribolium castaneum contained 117 CD36 protein sequences that were classified into five distinct branches on the evolutionary tree. Genes with similar structures were grouped together within the same branch, indicating a high conservation throughout evolution. CD36px4 and CD36px5 exhibit more intricate tertiary structures. The motifs motif19 and motif20 are exclusive to CD36px4, suggesting their potential involvement in species evolution. With a staggering 143 phosphorylation sites, CD36px4 may play a crucial role in the phagocytosis and clearance of apoptotic cells.【Conclusion】The 12 SCRs identified are transmembrane proteins with abundant phosphorylation sites, predicted to participate in various physiological processes such as intracellular and extracellular material transport, signal transduction, energy metabolism, and apoptosis regulation. They also possess the functions of mediating phagocytosis and clearance of cellular debris. These receptors represent a promising target for RNAi-based pesticides. The results have provided a crucial foundation for the screening of RNAi pesticide targets. |
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