| 董超,董丽英,薛静,等.云南布朗族地方稻种资源苗期稻瘟病抗性评价及抗性基因鉴定分析[J].广东农业科学,2025,(1-3):- |
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云南布朗族地方稻种资源苗期稻瘟病抗性评价及抗性基因鉴定分析 |
| Evaluation of blast resistance at seedling stage and identification of resistance genes in rice landraces of Bulang minority in Yunnan Province |
| 投稿时间：2024-06-07 修订日期：2024-12-29 |
| DOI： |
| 中文关键词: 地方稻种资源 布朗族 稻瘟病 抗性基因 功能标记 致病力 |
| 英文关键词: Rice Landraces Bulang minority Blast Resistance gene Functional marker Pathogenicity |
| 基金项目:云南省重大专项(202302AE090011)；云南省科技人才与平台计划(202405AD350066)；国家重点研发计划子课题(2021YFD1200505-1);云南省种子种业联合实验室项目(202205AR070001-11) |
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| 中文摘要: |
| 【目的】为明确农家保护下云南布朗族地方稻种资源稻瘟病抗性水平与分布。【方法】利用10个稻瘟病菌株和24个稻瘟病抗性基因的31个功能标记对78份地方稻种资源进行苗期稻瘟病抗性鉴定与分子检测。【结果】苗期接种鉴定结果表明:供试78份地方稻种中对10个菌株都表现为抗病的材料55份,占70.5%,且勐海县(99.0%)>墨江县(89.1%)>双江县(72.1%)。供试菌株08-16-6a-1和HN-09-1c-7对布朗族资源致病力最强(11.54%),而菌株HN-09-1c-7和08-20-1a-1对勐海县致病力最强(4.00%),菌株08-20-1a-1对墨江县资源致病力最强(36.36%),菌株08-16-6a-1对双江县资源致病力最强致病力最强(52.94%)。功能基因检测结果表明:布朗族地方稻种资源不含pi21、Pi25、Pi37、Pi36、Pi56、Pi63、Pi2/Pizt、Pib、Pid3、Pigm、Pik、Pikp1、Pi1、Pis、Pish、Pit和Pb1等18个基因,明确含有Pi9、Pi5、Pita、Pikh、Pid2和Pikm六个基因；抗性基因频率为73.08%-84.62%,最高的为Pid2,最低的为Pi9；勐海县中频率最高为Pita和Pid2,最低为Pi9；墨江县中频率最高为Pid2、Pi5和Pi9,最低为Pita；双江县中频率最高为Pid2、Pita、Pikm、Pikh和Pi9,最低为Pi5。【结论】农家保护下布朗族地方稻种资源抗性强、抗谱宽,筛选出苗期稻瘟病抗性好的资源55份,可作为稻瘟病抗性基因资源挖掘和利用；而抗性基因Pi9、Pi5、Pita、Pikh、Pid2、Pikm 在云南布朗族稻作生产上发挥重要作用。 |
| 英文摘要: |
| 【Objective】In order to determine the resistance level and distribution of rice blast On-farmers in Bulang minority of Yunnan Province.【Method】Rice blast resistance was identified and molecular detected in 78 rice landraces at seedling stage using 31 functional markers of 10 blast strains and 24 blast resistance genes.【Result】The results of inoculation identification at seedling stage showed that 55 of the 78 local rice varieties tested were resistant to 10 strains, accounting for 70.5%, and Menghai County (99.0%) > Mojiang County (89.1%) > Shuangjiang County (72.1%). Strains 08-16-6a-1 and HN-09-1c-7 showed the strongest pathogenicity against Bulang resources (11.54%), strains HN-09-1c-7 and 08-20-1a-1 showed the strongest pathogenicity against Menghai County (4.00%), and strain 08-20-1a-1 showed the strongest pathogenicity against Mojiang County (36.36%). Strain 08-16-6a-1 had the strongest pathogenicity against resources in Shuangjiang County (52.94%). The results of functional gene testing showed that: The rice landraces of Bulang people do not contain 18 genes, such as pi21, Pi25, Pi37, Pi36, Pi56, Pi63, Pi2/Pizt, Pib, Pid3, Pigm, Pik, Pikp1, Pi1, Pis, Pish, Pit and Pb1. It contains Pi9, Pi5, Pita, Pikh, Pid2 and Pikm. The frequency of resistance gene ranged from 73.08% to 84.62%, the highest was Pid2 and the lowest was Pi9. In Menghai County, the highest frequencies were Pita and Pid2, and the lowest frequencies were Pi9. In Mojiang County, the highest frequencies were Pid2, Pi5 and Pi9, and the lowest frequencies were Pita. In Shuangjiang County, the highest frequencies were Pid2, Pita, Pikm, Pikh and Pi9, while the lowest frequencies were Pi5.【Conclusion】The rice germplasm resources On-farm of Bulang minority had strong resistance and broad resistance spectrum. 55 rice germplasm resources with good resistance to rice blast at seedling stage were selected, which could be used as blast resistance gene resources. The resistance genes Pi9, Pi5, Pita, Pikh, Pid2,Pikm played an important role in the rice production of Bulang minority in Yunnan Province. |
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