| 曹华盛,李堂,熊亮,等.水稻磷脂酶D基因家族鉴定及表达分析[J].广东农业科学,2024,(12):- |
| Identification and Expression Analysis of the Phospholipase D GeneFamily in Rice |
| 投稿时间：2024-08-14 修订日期：2025-01-23 |
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| Abstract: |
| 【Objective】Phospholipase D (PLD) plays a crucial role in plant growth, development, and stress response. Explore the PLD gene family members involved in biotic and abiotic stress in rice (Oryza sativa L.) and investigate their biological functions.【Method】Identifying members of the rice PLD family based on the rice genome database, systematically analyzing the protein physicochemical properties, phylogenetics, conserved motifs, and cis-acting elements of the gene family members using bioinformatics methods. Analyzing the gene expression patterns of the family members under different biotic and abiotic stress conditions through RNA-seq data, and detecting the changes of total phospholipase D activity under stress treatment conditions.【Result】The rice PLD gene family has 17 members with amino acid numbers ranging from 512 to 1115 and relative molecular weights ranging from 57.19 to 123.82 kD; The isoelectric point of these protein are between 5.6 and 11. Between 23. According to the phylogenetic tree, the family is divided into four subfamilies, and there are significant differences in the number of exons among the members in different subfamilies; Chromosome localization analysis revealed that 17 PLD genes were located on 9 chromosomes. The analysis of cis-acting elements revealed that the promoter regions of PLD gene family members contain a large number of hormone responsive elements such as auxin, abscisic acid, and methyl jasmonate, as well as low temperature responsive, pressure defense, and anaerobic induction responsive elements; RNA-seq data analysis under biotic and abiotic stress conditions revealed that members of the PLD gene family were strongly induced by low nitrogen, ABA, and GA; In addition, enzyme activity analysis under biotic and abiotic stress conditions showed a significant increase of total phospholipase D activity after high salt stress and black stripe dwarf disease infection.【Conclusion】A total of 17 rice PLD genes were identified, and members in the same subgroup have similar conserved motifs. The expression of PLD gene family members in rice varies under different tissues and biotic and abiotic stress conditions. These results will provide assistance in understanding the role and mechanism of phospholipase D family in rice growth and development, biotic and abiotic stress response. |
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