| 邹芬,何烈干,罗向东,等.200份番茄材料6种病害9个抗病基因的分子标记检测[J].广东农业科学,2024,(12):- |
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200份番茄材料6种病害9个抗病基因的分子标记检测 |
| Molecular Marker Detection for 9 Disease Resistant Genes of 6 Diseases in 200 Tomato Germplasm |
| 投稿时间：2024-09-26 修订日期：2024-12-19 |
| DOI： |
| 中文关键词: 番茄 抗病基因 分子标记 抗病育种 检测 种质资源 |
| 英文关键词: tomato resistance gene molecular marker disease resistant breeding detection germplasm resources |
| 基金项目:江西省自然科学(20232BAB215036),江西省蔬菜产业技术体系资助项目(JXARS-06)和江西师范大学研究生创新(YJS2023063) |
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| 摘要点击次数: 19 |
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| 中文摘要: |
| 【目的】番茄生产上病害频发，给番茄产量和品质造成了严重影响。通过分子标记技术检测番茄上危害严重的6种病害的9个抗性基因，试图发掘番茄上多抗种质资源，从而为番茄抗病聚合育种提供理论依据。【方法】采用CTAB法提取番茄总DNA，根据已发表的引物对200份番茄材料分别进行灰叶斑病抗性基因Sm，叶霉病抗性基因Cf-5和Cf-9，番茄黄化曲叶病毒病抗性基因Ty-1、Ty-2和Ty-3/3a，根结线虫病抗性基因Mi，枯萎病抗性基因I-2和晚疫病抗性基因Ph-3的PCR扩增。【结果】200份番茄材料中，含灰叶斑病纯合抗性基因Sm的番茄材料共90份，杂合19份；叶霉病抗性基因中，含Cf-5的材料为12份，含Cf-9的材料为162份；番茄黄化曲叶病毒病抗性基因中，含纯合Ty-1的材料为33份、杂合17份，含纯合Ty-2的材料为7份、杂合1份，含纯合Ty-3/3a的材料为31份、Ty-3a/ty-3杂合20份；根结线虫病抗性基因中，含纯合Mi的材料为60份，杂合28份；枯萎病抗性基因中，含I-2的材料为75份；晚疫病抗性基因中，含纯合Ph-3的材料为25份，杂合5份。上述供试材料中，含3个或3个以上抗性基因的材料有126份，占比63%；其中含5个抗性基因的材料有19份；含6个抗病基因的材料有4份，分别为L36-1、Q9-1、Q9-2和TianF4，未检测到同时含7个及以上抗病基因的材料。【结论】200份番茄材料中有126份含3个及以上抗性基因，表明该批次番茄具有较好的应用潜力，尤其是材料L36-1、Q9-1、Q9-2和TianF4，均含6个抗性基因，可作为候选多抗种质资源进行下一步筛选鉴定。 |
| 英文摘要: |
| 【Objective】 The frequent occurrence of diseases in tomato production has seriously affected the yield and quality of tomato. Molecular maker technology was used to detect 9 resistance genes of 6 serious diseases in tomato, in an attempt to discover multi-resistant germplasm resources so as to provide theoretical basis for tomato resistance gene pyramiding strategy. 【Method】 Tomato total DNA was extracted by CTAB method, and the gray leaf spot resistance gene Sm, leaf mold resistance genes Cf-5 and Cf-9, tomato yellow curl virus disease resistance genes Ty-1, Ty-2 and Ty-3/3a, root knot nematode disease resistance gene Mi, fusarium wilt resistance gene I-2 and late blight resistance gene Ph-3 were amplified according to published primers on 200 tomato materials. 【Result】 Among 200 tomato materials, 90 materials contained homozygous resistance gene Sm, and 19 were heterozygous; among leaf mold resistance genes, Cf-5 was found in 12 materials, Cf-9 was found in 162 materials; among tomato yellow leaf curl virus resistance genes, 33 materials contained homozygous Ty-1 and 17 were heterozygous, 7 materials contained homozygous Ty-2 and 1 was heterozygous, 31 materials contained homozygous Ty-3/3a and 20 materials contained heterozygous Ty-3a/ty-3; among the root knot nematode resistance gene Mi, 60 materials were homozygous and 28 were heterozygous; as for fusarium wilt resistance gene, 75 materials contained I-2; among the late blight resistance gene Ph-3, 25 materials were homozygous and 5 were heterozygous. Among these tomato materials, there were 126 materials containing three or more resistance genes, accounting for 63% of the total, including 19 contained 5 genes, 4 contained 6 genes (line L36-1, Q9-1, Q9-2 and TianF4). None of the materials have 7 or more resistance genes.【Conclusion】 Among the 200 tomato materials, 126 contains 3 or more resistance genes, indicating that this batch of tomatoes have good potential for application, especially materials L36-1, Q9-1, Q9-2 and TianF4, all contains 6 resistance genes, which could be used as candidate multi-resistance germplasm resources for further screening and identification. |
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