| 叶楚,林蔚,谢炳春,等.中国辣椒ADH基因家族鉴定和表达分析[J].广东农业科学,2025,(2-3):- |
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中国辣椒ADH基因家族鉴定和表达分析 |
| Identification and Expression Analysis of the ADH Gene Family in Chinese Pepper |
| 投稿时间：2024-11-01 修订日期：2025-01-16 |
| DOI： |
| 中文关键词: 中国辣椒 乙醇脱氢酶 基因鉴定 表达分析 生物信息学 |
| 英文关键词: Capsicum chinense alcohol dehydrogenase gene identification expression analysis bioinformatics |
| 基金项目:国家重点研发计划(2023YFD1201502)；广东省农业厅种业振兴与科技兴农项目-高产优质辣椒新品种选育研究(2024-440000-87020100-8753)；广州市科技计划项目:多技术融合选育华南特色辣椒新品种(2023B03J1082)；毕节市科学技术揭榜挂帅项目-特色辣椒新品种选育及其高产栽培技术研究(毕科合重大专项〔2022〕3号) |
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| 中文摘要: |
| 【目的】中国辣椒(Capsicum chinense)为茄科(Solanaceae)辣椒属(Capsicum)植物,大多具浓郁果香,具有良好的商品性状。乙醇脱氢酶(Alcohol dehydrogenase)是是影响支链酯类物质含量的重要酶,催化相应的醇和醛之间的可逆转化,对支链酯类含量有重要影响。鉴定中国辣椒ADH基因家族成员并分析植株各部位表达模式,为后续功能研究提供参考。【方法】通过生物信息学分析和实时荧光定量PCR对中国辣椒ADH基因家族进行鉴定及表达特征分析。【结果】从中国辣椒基因组中鉴定到8个ADH基因,分布在4个染色体上,分别命名为CcADH1-8。理化性质预测结果显示,ADH基因家族编码的氨基酸序列长度在368~457aa之间,分子质量范围为40~49kDa,等电点范围为等电点5.50~9.12,亲水系数中CcADH4-6为正值,其余基因家族成员为负值。不稳定指数在22.23~44.81之间。蛋白质结构预测显示,CcADH的二级结构以α-螺旋和无规则卷曲为主,三级结构中CcADH4和CcADH6,CcADH7和CcADH8的结构相近。亚细胞定位预测发现CcADH均定位于细胞质中,其基因启动子上存在12种顺式调控元件。时空表达分析显示,CcADH6在辣椒植株各器官未检测到表达,CcADH7在花和果实第4时期高表达,CcADH2在根中高表达,CcADH8在花中高表达,CcADH1、CcADH4-6在果实发育第4时期高表达,CcADH3在果实发育第5时期高表达。【结论】明确中国辣椒ADH基因家族的表达模式,推测CcADH7可能是影响果实支链醇类物质含量的关键基因,为下游的AAT(醇酰基转移酶)提供特异底物,影响果实支链酯类物质含量。 |
| 英文摘要: |
【Objective】 Capsicum chinense is a member of the Solanaceae family and Capsicum genus, is known for its rich fruity aroma and good commercial traits. Alcohol dehydrogenase (ADH) is an important enzyme that affects the content of branched ester compounds, catalyzing the reversible transformation between corresponding alcohols and aldehydes, and has a significant impact on the content of branched esters. Identifying the members of the ADH gene family in Capsicum chinense ,and analyzing their expression patterns in different parts of the plant will provide a reference for subsequent functional studies. 【Method】 The ADH gene family in Capsicum chinense was identified and their expression characteristics analyzed through bioinformatics analysis and real-time fluorescent quantitative PCR. 【Results】Eight ADH genes were identified from the Capsicum chinense genome, distributed across four chromosomes and named CcADH1-8. Prediction of physicochemical properties showed that the amino acid sequence lengths of the ADH gene family encoded proteins range from 368 to 457aa, with molecular weights between 40 to 49kDa, and isoelectric points between 5.50 to 9.12. The hydrophilicity coefficients indicated that CcADH4-6 were positive, while the rest of the gene family members were negative. The instability indices ranged from 22.23 to 44.81. Protein structure prediction revealed that the secondary structure of CcADH was predominantly composed of α-helices and random coils, and CcADH4 and CcADH6, as well as CcADH7 and CcADH8, were similar in the tertiary structurer. Subcellular localization prediction showed that all CcADHs were localized in the cytoplasm, and their gene promoters contained 12 types of cis-regulatory elements. Spatiotemporal expression analysis showed that CcADH6 had no detectable expression, CcADH7 was highly expressed in flowers and the 4th stage of fruit development,CcADH2 was highly expressed in roots, CcADH8 was highly expressed in flowers, CcADH1, CcADH4-6 were highly expressed during the fourth stage of fruit development, and CcADH3 was highly expressed during the fifth stage of fruit development. 【Conclusion】Clarifying the expression patterns of the ADH gene family in Capsicum chinense,hypothesized that CcADH7 may be a key gene affecting the content of alcohol substances in the fruits, providing branched alcohols for downstream AAT enzymes, influencing the content of branched ester substances in the fruit.
Keywords:Capsicum chinense;alcohol dehydrogenase;gene identification; expression analysis;bioinformatics |
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