| 梅文宇,黎振兴,陈兴平,等.不同砧木嫁接番茄对酶活和激素含量的影响[J].广东农业科学,2024,(12):- |
| Effects of different rootstocks on enzyme activity and hormone content of grafted tomatoes |
| 投稿时间：2024-11-01 修订日期：2025-01-19 |
| DOI： |
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| 摘要点击次数: 81 |
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| Abstract: |
| 【Objective】 In order to study the affinity performance of grafting of solanaceous crops and clarify the physiological response at the interface of tomato scion grafted on different rootstocks. 【Method】 By setting tomato scion self-grafting ( TS / TS ) as a control, compared with tomato scion grafted tomato rootstock ( TS / TR ), tomato scion grafted eggplant rootstock ( TS / ER ), tomato scion grafted pepper rootstock ( TS / PR ), the changes of PPO, POD, SOD enzyme activity, total phenol, total flavonoids, malondialdehyde, auxin, cytokinin, gibberellin abscisic acid, jasmonic acid and salicylic acid content at different time points after grafting of different grafting combinations were systematically evaluated.【Result】The results showed that the content of total phenolic flavonoids and malondialdehyde at the interface of the grafting combination with better affinity was lower than that of other grafting combinations, the degree of oxidative stress at the interface was smaller, and the activity of antioxidant enzymes at the interface was also significantly lower than that of other combinations. The contents of total phenolic flavonoids, malondialdehyde and ABA in the interface of incompatible grafting combination ( TS / TR ) were significantly higher than those in other grafting combinations, and the interface oxidative stress was higher. PCA analysis of enzyme activity and related substance content at the interface showed that there were significant differences in interface oxidation related indexes between the same grafting and the different grafting. The determination of 6 hormones at the interface of different grafting combinations showed that the content of CTK was the lowest and the content of JA was the highest at the interface of self-grafting, and the content of CTK was the highest and the content of JA was the lowest at the interface of TS / PR combination. The correlation analysis of six hormones showed that JA and SA were significantly positively correlated at the interface of different grafting combinations, and CTK was significantly positively correlated with ABA. CTK was significantly negatively correlated with JA, ABA was significantly negatively correlated with JA, and SA was significantly negatively correlated with CTK.【Conclusion】This study found that in tomato grafted seedlings, the physiological and biochemical status of the interface is related to the grafting affinity. The better the rootstock-scion affinity, the lower the oxidative stress level of the interface. The worse the affinity, the higher the degree of oxidative stress in the combination. |
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