| 何凤婷,区翠仪,何广位,等.甘油法、砂土法及斜面法保藏虫生真菌效果比较[J].广东农业科学,2024,(3-4):- |
| A Comparative Research for Preservation of Entomogenous Fungi with Glycerin, Sand and PDA Slant |
| 投稿时间：2024-11-05 修订日期：2025-03-27 |
| DOI： |
|
| 摘要点击次数: 97 |
| 全文下载次数: 0 |
| Abstract: |
| 【Objective】 Entomogenic fungi are susceptible to strain degradation and reduced activity during the passage process. Selecting suitable strain preservation methods is a critical measure to reduce the passage of fungal culture in laboratory. 【Method】 In this study, three preservation methods, PDA slants, glycerin frozen, and sand tubes were employed to preserve 13 species of 18 strains of entomopathogenic fungi over periods of 3, 6, and 12 months. The preservation efficacy of the three different preservation methods were evaluated through comprehensive assessments including strain revival efficiency, mycelial growth rate, dehydrogenase activity, and determination of sporulation production. . 【Result】 At the 3-month preservation interval,, all three methods demonstrated satisfactory preservation effects, with the PDA slant method showing particularly favorable results. Among these, 15 strains exhibited higher mycelial growth rates and dehydrogenase activities, while 12 strains showed higher spore production compared to other methods, and all the strains had good mycelial growth and normal coloration. At the 6-month period, the glycerol frozen method demonstrated superior preservation efficacy. In this cohort, 14 strains exhibited higher mycelial growth rates, 15 strains showed higher spore production and all strains’ mycelial had better growth compared to other methods. Except for 6 strains in the PDA slant, the color of the other strains did not change. Among the mycelial dehydrogenase activities, 9 strains showed the best effect in the sand tube method. Over a 12-month period, the glycerol method yielded the best preservation effect, with minimal changes in colony color, mycelial growth rate, and spore production compared to the 3-month period. In the sand tube preservation method which has a storage period 12 months, Metarhizium robertsii MrGX0603, Beauveria bassiana BbGX7303, Purpureocillium lilacinum PiGX0201, Cordyceps cateniannulata CcGX32S01 and Cordyceps fumosorosea CfGX4206 demonstrated superior preservation outcomes. These strains showed higher dehydrogenase activity in the mycelium with recorded values of 1.18, 1.17, 1.13, 1.12, and 1.27 respectively, and better conidial production with recorded values of 53.50×106, 52.23×106, 62.00×106, 54.21×106, and 53.79×106 spores/cm2 respectively. At the 12 months mark, the mycelial growth rate, dehydrogenase activity and sporulation of the strain decreased slightly. The latter’s vitality of 18 strains had apparently decreased after 12 months of storage, and the effect of sand tubes preservation method was better than that of PDA slants. 【Conclusion】 Under identical preservation methods and timeframes, the preservation effects varied markedly among different strains. Different preservation methods can be selected according to the characteristics of the strain and storage period. The PDA slant preservation method is most suitable for short-term preservation of approximately 3 months. The glycerol method provided the best preservation effect for a 1-year storage period. The sand tube preservation method is more appropriate for strains with sporulation quantities exceeding 50×106 spores /cm2. |
|
View Fulltext
查看/发表评论 下载PDF阅读器 |
|
|
|
