| 安翠环,黄春贵,郭泽安,等.番茄GAD基因家族鉴定与生物信息学分析[J].广东农业科学,2025,(5-6):- |
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番茄GAD基因家族鉴定与生物信息学分析 |
| Identification and Bioinformatics Analysis of the GAD Gene Family in Tomato |
| 投稿时间：2024-11-12 修订日期：2025-08-12 |
| DOI： |
| 中文关键词: 番茄(solanum lycopersicum) GAD基因家族 生物信息学分析 蛋白结构 |
| 英文关键词: Tomato (Solanum lycopersicum) GAD gene family Bioinformatics analysis Protein structure |
| 基金项目:1.2023年中央引导地方科技发展资金项目(桂科ZY23055021)；2.2023年广西科技重大专项(桂科AA23062048)；3.广西农业职业技术大学科研项目合同(XKJ2301)； |
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| 中文摘要: |
| 【目的】植物谷氨酸脱羧酶（GAD）在γ-氨基丁酸（GABA）的生物合成中起关键作用，参与植物生长发育及逆境响应过程。番茄GAD基因家族的生物学功能及其分子机制尚不明确。该文旨在进一步解析番茄GAD基因的生物学功能，为其在 分子机制研究及遗传改良中的应用提供理论依据。【方法】通过生物信息学手段对番茄GAD基因家族成员进行包括染色体定位、基因结构、亲缘关系及组织特异性分析等在内的全面的生物信息学分析。【结果】番茄基因组包含5个GAD基因，分别位于1、3、4、5和11号染色体上，均具有典型的内含子-外显子结构。这些GAD蛋白均为不稳定蛋白，主要定位于细胞质，其二级结构以α-螺旋和无规则卷曲为主，活性中心及关键功能域进化上高度保守。番茄GAD基因与水稻、拟南芥同源基因序列相似性较高，但关键功能域存在特异性氨基酸变异。在广西野生番茄材料G173中，GAD基因在根、茎、叶中均未检测到表达，而在花和果实中表达存在显著差异，其中Solyc01G00002和Solyc04G001008表达量较高，Solyc03G002270在花和果实中的表达量接近。【结论】文中生物信息学分析方法可揭示番茄GAD基因家族在染色体分布、基因结构、蛋白性质、启动子元件和组织表达特异性等方面的特征，这些特征对于理解番茄GAD基因家族功能有重要意义。 |
| 英文摘要: |
| Abstract: 【Objective】Plant glutamate decarboxylase (GAD) plays a pivotal role in the biosynthesis of γ-aminobutyric acid (GABA), which is involved in plant growth, development, and stress responses. However, the biological functions and molecular mechanisms of the GAD gene family in tomato (Solanum lycopersicum) remain largely unexplored. This study aims to systematically characterize the tomato GAD gene family, including its genomic distribution, structural features, and evolutionary relationships. Through comprehensive expression profiling and functional analyses, we revealed the tissue-specific expression patterns of tomato GAD genes and their potential roles in fruit development and abiotic stress adaptation. These findings provide critical insights into the molecular mechanisms underlying GABA-mediated physiological processes in tomato and lay a theoretical foundation for future genetic improvement strategies targeting stress tolerance and fruit quality enhancement.【Method】Conduct a comprehensive bioinformatics analysis on the members of the tomato GAD gene family, including chromosome localization, gene structure, physicochemical properties of proteins, secondary structure, three - dimensional conformation, promoter analysis, phylogenetic relationships and tissue - specific expression analysis.【Result】The tomato genome contains five glutamate decarboxylase (GAD) genes localized to chromosomes 1, 3, 4, 5, and 11, all featuring canonical intron-exon structures. The encoded GAD proteins are unstable and predominantly cytoplasmic-localized, with α-helices and random coils constituting their dominant secondary structures; their active sites and functional domains are evolutionarily conserved. While tomato GAD genes show high sequence similarity to orthologs in rice (Oryza sativa) and Arabidopsis (Arabidopsis thaliana), specific amino acid variations exist within critical functional domains. In the native tomato accession G173 (Guangxi origin), GAD genes exhibited no detectable expression in roots, stems, or leaves but displayed significant tissue-specific expression differences in flowers and fruits: Solyc01g00002 and Solyc04g001008 showed relatively high expression, whereas Solyc03g002270 maintained comparable expression levels in both floral and fruit tissues.【Conclusion】The bioinformatics analysis provides valuable information about the chromosome distribution, gene structure, protein properties, promoter elements and tissue - specific expression of the tomato GAD gene family, which is crucial for understanding the function of this gene family. |
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