| 曹朝,陈晓峰.基于SSR技术分析烟台地区樱桃种质资源遗传多样性[J].广东农业科学,2025,(2-3):- |
| Analysis of Genetic Diversity of Cherry Germplasm Resources in Yantai Region Based on SSR Technology |
| 投稿时间：2024-11-13 修订日期：2025-03-18 |
| DOI： |
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| Abstract: |
| 【Objective】The genetic diversity and genetic relationship of cherry germplasm in Yantai area were analyzed, so as to provide a scientific basis for the identification and utilization of sweet cherry germplasm resources. 【Method】Thirty-one main sweet cherry varieties and 5 wild hairy cherry germplasms in Yantai area were used as test materials, and primers with good polymorphism were preliminarily screened by PCR amplification technology, and 36 cherry germplasms in Yantai were detected by simple sequence repeat (SSR) technology, and the DNA molecular ID card and fingerprint of cherry germplasm were constructed, and the genetic diversity and genetic structure cluster analysis were carried out. 【Result】Among the 36 cherry germplasms tested, 8 pairs of SSR primers were screened with good polymorphisms, a total of 73 alleles were detected, an average of 9.12 were amplified at each locus, the Shannon information index (I) was 1.25, the Nei's gene diversity index (H) was 0.58, and the polymorphism information content (PIC) was 0.55 on average, indicating that the cherry germplasm in Yantai area had significant genetic differences and rich genetic diversity. The SSR amplification bands were analyzed and encoded, and the molecular ID cards and fingerprints of 36 cherry germplasms were successfully constructed. The UPGMA method was used to construct a cluster analysis map, and the 36 cherry germplasms were divided into two categories: sweet cherry and wild hairy cherry at the genetic coefficient of 0.170, indicating that the genetic relationship between wild hairy cherry and sweet cherry germplasm was obviously distant, and at the genetic coefficient of 0.556, the 36 cherry germplasms could be divided into 7 categories, and the r-value was 0.93, and the analysis results were consistent with the fingerprint. For the 31 sweet cherry germplasms in Yantai area, the genetic coefficient was 0.611, which could be redivided into 5 categories, and the clustering results were in good agreement with the clustering of categories based on agronomic morphological characteristics and ripening season. 【Conclusion】 The primer polymorphisms of the selected primers were good, and they could be preferred in the genetic diversity analysis, fingerprint construction and cluster analysis of cherry germplasm. The results of this study can provide strong support for the identification of sweet cherry germplasm and the conservation of genetic diversity in Yantai area, and provide an important reference for its breeding selection and genetic structure analysis. |
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