| 戴兴鑫,刘艳洁,李成霞,等.利用野生稻单片段代换系定位株高性状QTLs[J].广东农业科学,2024,(11):- |
| QTL Mapping of Plant Height in Single-segment Substitution Lines of Wild Rice |
| 投稿时间：2024-12-16 修订日期：2024-12-31 |
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| 摘要点击次数: 85 |
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| Abstract: |
| Abstract: 【Objective】Plant height is an agronomic trait closely related to rice plant architecture, which has an important role in rice yield. Through fully exploring the elite genetic loci for plant height in wild rice, it would be helpful in the enrichment of dwarf genes and provide new genetic resources for breeding rice varieties with ideal plant height.【Method】In this study, two sets of single-segment substituted lines, MER-SSSLs from Oryza meridionalis and BAR-SSSLs from Oryza barthii, were used as materials, trait of plant height were investigated through multi-seasons’ field trials. To detect QTLs controlling plant height in SSSLs, the different significance between the SSSLs and the recipient parent, ‘HJX74’, were analyzed by Duncan's multiple comparison test.【Result】The plant heights of the MER-SSSLs and BAR-SSSLs populations showed a unimodal distribution in all three seasons with a wide variation range, the range was from 80.74 cm to 116.22 cm and 80.26 cm to 167.33 cm, respectively. A total of 25 QTLs were identified in MER-SSSLs during three cropping seasons, the additive effects of the QTLs ranged from -6.07 cm to 6.35 cm, and the phenotypic variation explained by individual QTL was from 2.34% to 6.63%. Among them, seven QTLs (qPHm2-1, qPHm2-2, qPHm3-1, qPHm4-2, qPHm5-1, qPHm5-4, and qPHm5-5) were identified repeatedly in different seasons, presenting as the stable QTLs. Importantly, qPHm2-1, qPHm2-2, qPHm3-1, qPHm4-2, and qPHm5-4 have not been reported previously, which were considered novel QTLs of plant height in MER-SSSLs. In BAR-SSSLs population, a total of 21 QTLs were identified in late season of 2024, the additive effects ranged from -5.41 cm to 29.28 cm, with the phenotypic contributions from 2.45% to 30.17%. It found both qPHb1-4 and qPHb10-1 showed the phenotypic contributions of more than 18%. In addition, qPHb10-1 might be a novel QTL identified in BAR-SSSLs.【Conclusion】Plant height is a quantitative trait controlled by multiple genes in rice. Seven stable QTLs for plant height were identified in SSSLs of Oryza meridionalis and two main QTLs in SSSLs of Oryza barthii. It lays the foundation for further cloning of the elite genes for plant height in wild rice, and provides valuable genetic resources for breeding rice varieties with ideal plant height. |
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