| 牟佳美,李成霞,汤睿,等.野生稻SSSLs群体穗伸出度评价及长穗颈QTLs挖掘[J].广东农业科学,2024,(12):- |
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野生稻SSSLs群体穗伸出度评价及长穗颈QTLs挖掘 |
| Evaluation of Panicle Exsertion and Identification of QTLs for Long Panicle Neck in SSSLs Population of Wild Rice |
| 投稿时间：2024-12-16 修订日期：2025-01-06 |
| DOI： |
| 中文关键词: 野生稻 穗伸出度 单片段代换系 代换作图 QTL 加性效应 |
| 英文关键词: panicle exsertion single-segment substitution lines(SSSLs) substitution mapping QTL additive effect value |
| 基金项目:广东省自然科学基金（2024A1515010417，2019A1515010820）；广州市科技计划项目（201804020086） |
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| 中文摘要: |
| 【目的】水稻抽穗时出现严重的包穗，是影响产量的重要因素之一。而野生稻普遍具有良好的穗伸出度，本团队前期以南方野生稻和短舌野生稻为供体构建了单片段代换系（SSSLs）群体，本研究拟挖掘稳定表达的长穗颈QTLs，为野生稻长穗颈基因克隆及水稻穗伸出度性状的分子育种奠定基础。【方法】以P<0.01为差异显著的标准，筛选出穗伸出度显著长于HJX74的SSSLs，认为其代换片段携带控制长穗颈的QTL，并计算其加性效应及表型贡献率。【结果】在南方野生稻SSSLs鉴定到4个稳定QTLs（qPEm5-1、qPEm5-2、qPEm8-1、qPEm10-1），其加性效应均值范围为1.64 cm~2.19 cm，加性效应表型贡献率范围为114.53%~347.98%。在短舌野生稻SSSLs鉴定到11个稳定QTLs（qPEb1-1、qPEb1-2、qPEb1-3、qPEb2-1、qPEb3-1、qPEb3-2、qPEb4-1、qPEb5-1、qPEb6-1、qPEb7-1和qPEb10-1），其加性效应均值范围为1.74 cm~4.08 cm，加性效应表型贡献率范围为107.82%~481.80%。从以上两种野生稻SSSLs鉴定的长穗颈QTLs中发现qPEm1-1与qPEb1-1、，qPEm1-1与qPEb7-1、qPEm10-1与qPEb10-2有重叠区间，表明2个供体野生稻的重叠区间存在相同QTLs或等位基因。此外，本研究挖掘到3个新的QTLs（qPEm5-1、qPEb5-1、qPEb6-1），且其增效等位基因来自供体野生稻。【结论】本研究对以南方野生稻和短舌野生稻为供体构建的SSSLs群体进行了多季节的穗颈长性状调查，鉴定出15个稳定表达的长穗颈QTLs，其中3个QTLs未见报道。研究结果为进一步的长穗颈基因克隆与功能解析、以及水稻穗颈长性状的分子育种提供了参考与重要材料。 |
| 英文摘要: |
| 【Objective】The phenomenon of panicle enclosure in rice is one of the important factors affecting the grain yield. Panicle exsertion of the wild rice species is generally in good state. In this study, by using the single-segment substitution lines (SSSLs) of Oryza meridionalis and O. barthii developed in our laboratory, the trait of panicle exsertion was investigated and the QTLs controlling panicle exsertion were identified, in order to lay a foundation for further cloning of the elite genes for panicle exsertion from wild rice,and to provide the genetic resources for the molecular breeding of the panicle exsertion in rice.【Method】Taking P < 0.01 as the criterion for significant difference, the SSSLs with significant longer panicle neck than that of HJX74 were screened out. Meanwhile, the controlling QTLs located in the interval of the substituted segments were identified, and their additive effects and phenotypic contribution rates of the QTLs were evaluated.【Result】A total of 4 stably expressed QTLs (qPEm5-1, qPEm5-2, qPEm8-1, and qPEm10-1) were identified in the SSSLs population of O. meridionalis. The average additive effect values were in the range of 1.64 cm~2.19 cm, respectively, and the phenotypic contribution rates of the additive effects were in the range of 114.53%~347.98%. In addition, a total of 11 stably expressed QTLs (qPEb1-1, qPEb1-2, qPEb1-3, qPEb2-1, qPEb3-1, qPEb3-2, qPEb4-1, qPEb5-1, qPEb6-1, qPEb7-1, and qPEb10-1) were identified in the SSSLs population of O. barthii. Their average additive effect values were in the range of 1.74 cm~4.08 cm, respectively, and the phenotypic contribution rates of the additive effects were 107.82%~481.80%. Among the QTLs of the two wild rice species, it was found that qPEm1-1 and qPEb1-1, qPEm1-1 and qPEb7-1, and qPEm10-1 and qPEb10-2 had the overlapping interval, respectively. It was implied that there probably have the same QTLs or alleles controlling the long panicle neck in the overlapping intervals of the two donor wild rice. Moreover,three QTLs, qPEm5-1, qPEb5-1, and qPEb6-1 were considered as novel QTLs in this study, and their favorable alleles with positive effects were from the donor wild rice.【Conclusion】In this study, the panicle exsertion of the SSSLs populations of O. meridionalis and O. barthii were investigated in multiple seasons, and a total of 15 stably expressed QTLs for long panicle neck were identified. Among them, 3 novel QTLs were found. The results will lay a foundation for both further cloning and functional analysis of panicle exsertion genes from wild rice and molecular breeding for the panicle exsertion in rice. |
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