| 郭 义,李鹏燕,李敦松.荔枝蒂蛀虫不同地理种群遗传多样性的初步研究[J].广东农业科学,2024,(4-6):- |
| Preliminary study on genetic diversity of different geographical populations of the litchi fruit borer, Conopomorpha sinensis (Bradly) |
| 投稿时间：2024-01-13 修订日期：2024-02-28 |
| DOI： |
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| Abstract: |
| 【Objective】To clarify the genetic structure characteristics, genetic diversity level and molecular variation of different geographic populations of Conopomorpha sinensis, so as to provide scientific evidence for the study of its origin and evolution, and provide reference information for the control of this pest in different regions.【Method】Using the C. sinensis from 5 southern production areas of our country as materials, Genotyping the samples using GBS (Genotyping-by-sequencing) technology, then identifying SNP locus with SAMTOOLS software, according to SNP genotyping results, phylogenetic tree was constructed by the adjacency method. Principal component analysis was performed.【Result】Using GBS simplified genome sequencing technology, we sequenced C. sinensis in 5 regions. A total of 27.39 Gb of high quality effective data were obtained, with an average of 5.48 Gb per sample. A total of 11 767 high quality single nucleotide polymorphism (SNPs) locus were obtained. The results of principal component analysis showed that C. sinensis was divided into 2 groups in 5 regions, and the Luzhou population in Sichuan was significantly different from the other 4 populations. Phylogenetic tree analysis showed that Luzhou population was far from other populations. Both principal component analysis and phylogenetic tree analysis revealed that there were obvious differences in genetic level among C. sinensis of different geographical origin. The results of genetic diversity analysis showed that the observed heterozygosity was 0.2099~0.2531, the expected heterozygosity was 0.1937~0.2203, π was 0.2495~0.2748, indicating that the genetic diversity level of C. sinensis population was low. The results of AMOVA analysis showed that the genetic variation between populations (0.87%) was smaller than that within populations (99.13%), indicating that the variation mainly came from within populations rather than between populations.【Conclusion】 The genetic diversity level of C. sinensis in different geographic populations was low, the genetic differentiation degree of Luzhou population in Sichuan was high, and the genetic relationship with other populations was distant. |
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