| 刘思嘉,杨荣超.番茄组蛋白修饰基因家族鉴定及表达分析[J].广东农业科学,2024,(4):- |
| Identification and expression analysis of histone modification gene families in tomato |
| 投稿时间：2024-02-05 修订日期：2024-04-06 |
| DOI： |
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| 摘要点击次数: 47 |
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| Abstract: |
| Abstract:【Objective】Functional modifications of plant histones, including acetylation modification and methylation modification, play important roles in plant growth and development and in response to adversity. The biological functions and molecular mechanisms of the histone modification (HM) gene family in tomato are still unclear. The present study aims to further clarify the biological functions of the HM genes in tomato, and to lay the foundation for the study of their molecular mechanisms and genetic improvement of tomato..【Method】We identified tomato HM members based on the tomato genome database, systematically analyzed the phylogeny, gene structure, and chromosome localization of its HM gene family members using bioinformatics methods, and analyzed the spatiotemporal and temporal expression patterns of the tomato HM gene family through an online database.【Result】A total of 148 tomato HM genes were identified in this study, of which 32 encoded histone acetyltransferases (HATs), 11 encoded histone deacetylases (HDACs), 50 encoded histone methyltransferases, and 55 encoded histone demethylases (HDMs); the 148 genes were unevenly distributed on different chromosomes, of which 55 encoded histone demethylases (HDMs). methyltransferases (HMTs), and 55 genes encoding histone demethylases (HDMs); 148 genes were unevenly distributed on different chromosomes, with the highest number of genes on chromosome 3 (21 genes) and the lowest number of genes on chromosome 12 (4 genes). Gene structure characterization showed that the exons varied greatly among tomato HM genes, with the most exons amounting to 34 and the least to only 1. Protein structural domain analysis showed that Solyc07g064130, Solyc11g005670, and Solyc10g006480 contained only the Ubl structural domains, Solyc07g062100, Solyc10g077110 and Solyc03g083310 contain only the Zn-finger structural domain, another 10 genes contain the Bromo structural domain, 48 genes contain only the presence of the SET structural domain, and other genes contain other structural domains such as PLN02529 and PRMT5. In addition, the tomato HM genes were distantly related to the homologous proteins of this class in both Arabidopsis and rice, and the protein sequences of the tomato HM genes were more closely related to those of the Arabidopsis homologs than to those of rice. Based on phylogenetic clustering analysis, the tomato HM family genes were divided into four subfamilies, HAT, HDAC, HMT, and HDM. Tissue expression analysis showed that the HDAC subfamily was highly expressed in flowers (F30) and immature fruits (Plgfr) at 30 d after germination; the HAT subfamily was highly expressed in flowers (F30) at 30 d after germination, in fruits (10DPA) at 10 d after flowering, in flowers (fr), in roots (rr), and in immature fruits (Plgfr); the HDM subfamily was highly expressed in flowers (F30) at 30 d after germination, in fruits at 50 d after flowering, and in fruits at 50 d after flowering. flowers (F30), at 50% of flowers reaching anthesis (F45), flower buds (br), flowers (fr), roots (rr), 3 cm fruits (3fr), and immature 5 cm fruits (PB+5fr); the HDM subfamily was highly expressed in flowers 30 d after germination (F30), at 50% of flowers reaching anthesis (F45), flower buds (br), 3 cm fruit (3fr), and immature 5 cm fruit (PB+5fr).【Conclusion】The gene structures of different tomato HMs vary greatly and contain multiple protein functional structural domains. The homologous genes of tomato HM are distant from those of Arabidopsis thaliana and rice, and are expressed in different growth and developmental stages of plants and in different organ tissues, suggesting that this class of genes may be involved in a variety of growth and developmental processes in tomato. |
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