| 陈静,王重荣.稻瘟病抗性基因Pi2、Pita的特异KASP标记的开发与应用[J].广东农业科学,2024,(5-6):- |
| Development and application of specific KASP markers for rice blast resistance genes Pi2 and Pita |
| 投稿时间：2024-03-10 修订日期：2024-03-21 |
| DOI： |
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| 摘要点击次数: 32 |
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| Abstract: |
| 【Objective】The rice blast resistance genes Pi2 and Pita are two genes with broad-spectrum resistance to rice blast physiological races in south China, which have very important application value for rice blast resistance breeding in rice, so the development of a set of efficient identification methods is conducive to genetic breeding and variety improvement.【Methods】 This study according to the high resistance to rice blast varieties yellow oil and high sense rice blast varieties wide dwarf 4 in Pi2 gene 787,788 codon variation GCA GGA / GTG TTA, and high resistance international rice germplasm resources Tetep and high sense of Lijiang new black valley in the 6640th base variation G / T, based on the competitive allele PCR (Kompetitive Allele Specific PCR, KASP) labeling technology principle,【Results】 Using the KASP functional molecular markers developed in this study, we can detect the allele genotypes of the rice blast resistance genes Pi2 and Pita in the breeding materials in the early stage (seed or seedling stage), predict the rice blast resistance in the breeding materials, and do not need to identify the breeding materials in the disease garden.【Conclusion】Two functional loci of rice blast resistance gene KASP markers can well distinguish the different resistance of parental varieties, also clearly distinguish different allele genotypes in breeding materials, screening identified single strains carrying resistance alleles, used for subsequent cultivating good varieties, shows that functional KASP markers can promote resistance genetic improvement of rice varieties, improve the efficiency of breeding varieties. |
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