| 邱道寿,罗永坚,李长发,等.骨碎补大棚栽培品与野生品的广靶代谢组学比较分析[J].广东农业科学,2024,(5):- |
| Comparative analysis of metabolomics between greenhouse cultivated and wild collected Rhizoma Drynariae |
| 投稿时间：2024-02-19 修订日期：2024-03-09 |
| DOI： |
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| 摘要点击次数: 67 |
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| Abstract: |
| 【 Objective 】 Rhizoma Drynariae is a perennial traditional Chinese medicinal herb. Currently, most of the medicinal materials come from wild collection, and there are few reports of artificial cultivation. Due to the serious collection and destruction of its wild resources, medicinal resources have sharply decreased, and artificial cultivation has become an inevitable trend. On the basis of attempting greenhouse cultivation techniques for Rhizoma Drynariae, this study utilized metabolomics techniques to conduct targeted metabolomics comparative studies between greenhouse cultivated materials and wild collected materials, in order to provide theoretical basis for the artificial cultivation techniques and cultivation effects of Rhizoma Drynariae.【 Method 】This study focuses on the original plant of Rhizoma Drynariae, Drynaria fortune, and uses UPLC-MS/MS analysis technology to compare and analyze the differential metabolites between greenhouse cultivated three-year rhizomes and its wild three-year rhizomes. Subsequently, enrichment pathway analysis of differential metabolites was conducted using the KEGG pathway database. 【 Result 】 Out of 749 metabolites detected from two habitat samples, 100 differential metabolites were screened. Compared with wild samples, there were 58 upregulated metabolites and 42 downregulated metabolites in greenhouse cultivation samples. These differential metabolites mainly include flavonoids, organic acids, amino acids and their derivatives, phenolic acids, alkaloids, free fatty acids, and other metabolites. Among them, in greenhouse cultivation samples, flavonols, flavonoid glycosides, sugars, and carbohydrate metabolites were all upregulated, while in wild samples, amino acids and derivatives, phenolic acids, lysophosphatidylcholine, and anthocyanin metabolites were more upregulated. Further pathway enrichment analysis showed that 100 differential metabolites were annotated into 47 metabolic pathways, among which significantly enriched pathways included vitamin B6 metabolism, astragalus compound biosynthesis, diarylheptane biosynthesis, gingerol biosynthesis, flavonoid and flavonol biosynthesis, cysteine and methionine metabolism, anthocyanin biosynthesis, and aminoacyl tRNA biosynthesis.【Conclusion】 The content of flavonols, flavonoid glycosides, sugars, and carbohydrate metabolites in greenhouse cultivated Rhizoma Drynariae is significantly higher than that in wild cultivated Rhizoma Drynariae. Considering that the total flavonoids of Rhizoma Drynariae are recognized as the main active ingredients, greenhouse cultivated Rhizoma Drynariae has the advantage of main active ingredients compared to wild cultivated Rhizoma Drynariae, which can provide scientific basis for large-scale facility cultivation or artificial cultivation of Rhizoma Drynariae. |
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