文章摘要
董 博 1 ,邓涵逸 1 ,孙艺秋 1 ,房元杰 1 ,彭淼曦 1 ,石 琼 2,3, 贝锦龙 1 ,魏文康 1 ,陈 庄 1.基于肠道宏基因组对弹涂鱼两栖适应性的研究[J].广东农业科学,2022,49(11):32-42
查看全文    HTML 基于肠道宏基因组对弹涂鱼两栖适应性的研究
Study on the Amphibious Adaptability of MudskippersBased on Intestinal Metagenome
  
DOI:10.16768/j.issn.1004-874X.2022.11.004
中文关键词: 胃肠道微生物群  宏基因组学  两栖弹涂鱼  陆地适应  免疫学特异性
英文关键词: gastrointestinal microbiome  metagenomics  amphibious mudskipper  terrestrial adaptation  immnological specificity
基金项目:华南应用微生物国家重点实验室开放基金(SKLAM002-2021 );华南地区乡村振兴专项资金 (2021KJ382);茂名实验室科研启动项目(2021TDQD002);华南地区农业科学院地方分院和专家工作站工作经费 项目(2022 支撑 11)
作者单位
董 博 1 ,邓涵逸 1 ,孙艺秋 1 ,房元杰 1 ,彭淼曦 1 ,石 琼 2,3, 贝锦龙 1 ,魏文康 1 ,陈 庄 1 1. 广东省农业科学院农业生物基因研究中心 / 广东省农作物种质资源保存与利用重点实验室 广东 广州 5106402. 深圳市海洋生物基因组 学重点实验室广东 深圳 518083 3. 深圳大学生命科学与海洋学院广东 深圳 518061 
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中文摘要:
      【目的】从基 因组水平上报道两种有代表性的两栖鱼类大弹涂鱼(Boleophthalmus pectinirostris, BP)和大鳍弹涂鱼(Periophthalmus magnuspinnatus,PM)对陆地生活的适应性,以揭示胃肠道微生物群对弹涂 鱼在陆地生活的适应性及其特殊免疫力具有关键作用。【方法】采用 16S 扩增子测序和宏基因组技术,利用两种 有代表性的弹涂鱼和 3 种典型水生鱼类(草鱼 Ctenopharyngodon idellus、鲢鱼 Hypophthalmichthys molitrix 和鳙鱼 Aristichthys nobilis)的宏基因组数据,比较两栖鱼类和水生鱼类肠道的微生物组成、多样性、丰度和功能,并研 究潜在陆生标志微生物群。此外,通过查阅大量文献得到具有代表性的海水鱼类、淡水鱼类、两栖动物和陆生动 物的胃肠道微生物群数据,比较弹涂鱼的肠道菌群组成和功能。【结果】在弹涂鱼的胃肠道微生物群中,厚壁菌门、 变形菌门、拟杆菌门和梭杆菌门占主导地位。这些菌的含量在大鳍弹涂鱼、大弹涂鱼和水生鱼类之间存在显著差 异。草鲢鳙和弹涂鱼二者胃肠道微生物群之间最大的区别是 CKC4 门(一种推测与宿主脂质代谢相关的胃肠道细 菌门类)的比例,草鲢鳙中该比例从 4% ~27%不等,而在弹涂鱼中则没有发现。此外,弹涂鱼的胃肠道微生物 群中同时具有陆生动物、淡水和海水鱼类以及两栖动物的典型细菌家族,这与它们在水陆过渡咸淡水交界处的生 活特征相吻合。与陆生动物相比,鱼类拥有更高比例的梭状杆菌和变形杆菌,而陆生动物则具有更多的拟杆菌。 在大弹涂鱼和大鳍弹 涂鱼中还发现了此前被认为仅存在于陆生动物的 S24-7 菌株。【结论】推测弹涂鱼胃肠道微 生物群落相比水生性鱼类具有复合和多样性特征,可以通过病原体相关分子模式 PAMP 刺激宿主在进化中形成多 样性更高、拷贝数更多的天然免疫受体基因家族,以此适应更为复杂的两栖生活环境。
英文摘要:
      【Objective】In this study, we report the adaptability of two representative amphibian species, blue-spotted mudskipper (Boleophthalmus Periophthalmodon, BP) and giant-fin mudskipper (Periophthalmus magnuspinnatus, PM) to live on land , with a view to revealing the key role of gastrointestinal microbiota in the adaptation and specific immunity of mudskippers to amphibious life. 【Method】By using 16S amplicon sequencing and metagenomic tec hniques, the microbial composition, diversity, abundance and function of the intestines of amphibian and aquatic fish species were compared. The metagenomic data were utilized to compare two representative mudskippers (BP, PM) and three typical aquatic fish species including Ctenopharyngodon idella (CI), Hypophthalmichthys molitri (HM) and Aristichthys nobilis (AN) and the potential terrestrial marker gastrointestinal microbiota in mudskippers were investigated. In addition, the representative data of gastrointestinal microbiota from marine fish, freshwater fish, amphibians and terrestrial animals were obtained through extensive literature to compare the composition and function of intestinal microbiota in mudskippers.【Result】Firmicutes, Proteobacteria, Bacteroidetes and Fusobacteria were dominant in the gastrointestinal microbiota of mudskippers. The contents of various dominant phyla were strikingly different among BP, PM and aquatic fishes. Th e most significant difference in the gastrointestinal microbiota between the two groups was the proportion of CKC4, a gastrointestinal bacterial phylum that is speculated to be related to host lipid metabolism, which ranged from 4% to 27% in CI, HM, AN but was not found in the mudskipper. In addition, the gastrointestinal microbiota of mudskipper containeds typical bacterial families in terrestrial animals, freshwater and seawater fishes fish and amphibians, which was consistent with their life characteristics at the salt-water interface between water and land. It was also observed that fishes had a higher proportion of Clostridium and Proteobacteria than terrestrial animals, which had more Bacteroides species. More interestingly, certain bacteria strains like S24-7, previously thought to be specific in terrestrial animals, were also identified in both BP and PM. 【Conclusion】 The results suggest that the gastrointestinal microbial communities of mudskipper are more complex and diverse than those of aquatic fishes, which subsequently stimulate the host to form innate immune receptor gene families with higher diversity and more copy numbers through pathogen-related molecular pattern (PAMP) to a more complicated amphibian environment.
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