文章摘要
曹宗喜,潘全会,林哲敏,孔留五,何逸民,张桂红.PRRSV广东株XH-GD的分离及其NSP2、ORF3、ORF5的序列分析[J].广东农业科学,2013,40(12):159-163
查看全文    HTML PRRSV广东株XH-GD的分离及其NSP2、ORF3、ORF5的序列分析
Isolation and sequence analysis of NSP2, ORF3 and ORF5 of PRRSV XH-GD strain from Guangdong province
  
DOI:
中文关键词: 猪繁殖与呼吸综合征病毒  XH-GD毒株  序列分析
英文关键词: Porcine reproductive and respiratory syndrome(PRRSV)  XH-GD isolation  sequence analysis
基金项目:国家自然科学基金(31272564)曰国家自然科学基金会(NSFC)-广东联合基金项目(U0931003)曰国家现代农业产业技术体系专项(CARS-36)曰海南省科学事业费项目(琼财预[2013]131 号)
作者单位
曹宗喜,潘全会,林哲敏,孔留五,何逸民,张桂红 海南省农科院畜牧兽医研究所/海南省热带动物繁育与疫病研究重点实验室华南农业大学农业部兽用疫苗创制重点实验室 
摘要点击次数: 1525
全文下载次数: 570
中文摘要:
      将采集的广东某猪场疑似猪高热症病料处理后接种于Marc-145细胞,出现细胞聚集、固缩、脱落等特异性细胞病变。根据GenBank中猪繁殖与呼吸综合征病毒(PRRSV)的基因序列设计并合成针对NSP2、ORF3和ORF5基因的引物,扩增目的基因并测序分析。结果表明:分离毒株XH-GD株PRRRSV属于美洲型,且NSP2基因发生30个氨基酸的不连续缺失;XH-GD的NSP2、ORF3和ORF5基因与HUB1、NX06尧BJsy06、VR-2332、HB-1(sh)2002、HB-2(sh)2002、CH-la和RespPRRS MLV等毒株等位基因的核苷酸同源性分别为83.3%~98.9%、88.6%~99.2%、88.1%~99.2%,推导的氨基酸同源性分别为76.8%~98.3%、83.7%~98.8%、88.1%~99.2%;而与LV的等位基因核苷酸同源性分别为52.9%、65.0%和64.3%,推导的氨基酸同源性分别为31.3%、57.5%和58.9%。基因系统发育树分析表明,XH-GD与NX06、BJsy06株的亲缘关系很近,与HUB1、NX06、BJsy06、VR-2332、HB-1(sh)2002、HB-2(sh)2002、CH-la和RespPRRSMLV亲缘关系较近,与LV毒株处于不同的分支。
英文摘要:
      The samples collected from Guangdong province were inoculated to Marc-145 cell, the PRRSV specific cytopathogenic effect (CPE) was observed and the isolates named XH-GD obtained. After reverse transcription polymerase chain reaction, the amplified NSP2、ORF3 and ORF5 fragments were analyzed by sequence comparison. The results demonstrated that XH-GD belonged to the North American genotype and its NSP2 had 30 amino acids deletion. The NSP2, ORF3 and ORF5 of XH-GD shared 83.3%~98.9%, 88.6%~99.2% and 88.1%~99.2% of nucleotide similarity, and 76.8%~98.3%, 83.7%~98.8% and 88.1%~99.2% of deduced amino acid similarity, with that of HUB1, NX06, BJsy06, VR-2332, HB-1 (sh)2002, HB-2 (sh)2002, CH-la and RespPRRS MLV respectively; and shared only 52.9%, 65.0% and 64.3% of nucleotide similarity, and 31.3%, 57.5% and 58.9% of deduced amino acid similarity with that of LV respectively. The phylogenetic tree revealed XH-GD had closer relationship with HUB1, NX06, BJsy06, VR-2332, HB-1(sh)2002, HB-2(sh)2002, CH-la and RespPRRS MLV strains than that of LV strain.
  查看/发表评论  下载PDF阅读器

手机扫一扫看