文章摘要
胡会刚,叶自行,胡桂兵,董晨.应用RAPD标记构建柑桔分子遗传图谱[J].广东农业科学,2013,40(18):123-126
查看全文    HTML 应用RAPD标记构建柑桔分子遗传图谱
Construction of citrus genetic linkage map based on RAPD markers
  
DOI:
中文关键词: 柑桔  遗传图谱  RAPD
英文关键词: citrus  genetic linkage map  RAPD
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作者单位
胡会刚,叶自行,胡桂兵,董晨 1.中国热带农业科学院南亚热带作物研究所/农业部热带果树生物学重点实验室广东湛江5240912.华南农业大学园艺学院广东广州510642 
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中文摘要:
      以清见柑桔为母本、台湾椪柑为父本杂交获得的F1群体以及两个亲本作为作图群体,利用RAPD分子标记构建了清见和台湾椪柑的分子遗传图谱。母本清见的30个分离位点形成7个连锁群,覆盖总图距328 cM,位点之间平均距离为10.93 cM,连锁群的平均长度为47 cM,每个连锁群平均包含4.3个位点;父本台湾椪柑的23个位点形成(9+1)个连锁群,覆盖总图距317 cM,位点之间的平均遗传距离为13.78 cM,连锁群的平均长度为31.7 cM,每个连锁群平均包含2.3个位点。
英文摘要:
      A high-resolution genetic linkage map of citrus was constructed by a compatible mating isolated from the “Kiyomi”Χ“Taiwan Ponkan”using Random-Amplified Polymorphic DNA(RAPD). The linkage maps of “Kiyomi”Χ “Taiwan Ponkan”were calculated and constructed. The molecular genetic map of pistillate parent, “Kiyomi”, showed 30 separated loci containing 7 linkage groups which covered 328 cM total map distance with average distance of 10.93 cM between every two loci. The linkage groups displayed an average length of 47 cM and average 4.3 loci in every linkage group. On the other hand, the molecular genetic map of pollen parent, “Taiwan Ponkan”, showed 23 loci containing 9 plus 1 linkage groups which covered 317 cM total map distance with average genetic distance of 13.78cM between every two loci. The linkage groups displayed an average length of 31.7 cM and average 2.3 loci in every linkage group.
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