文章摘要
邵秀红,马柱文,袁清华,李集勤,潘晓英,黄振瑞.烟草种质资源重要活性成分含量差异研究[J].广东农业科学,2021,48(12):91-99
查看全文    HTML 烟草种质资源重要活性成分含量差异研究
Study on Content Differences of Important Active Ingredients in Tobacco Germplasm Resources
  
DOI:10.16768/j.issn.1004-874X.2021.12.011
中文关键词: 烟草  种质资源  活性成分  绿原酸  芦丁  茄尼醇
英文关键词: tobacco  germplasm resource  active ingredient  chlorogenic acid  rutin  solanesol
基金项目:广东省农业科学院作物研究所所长基金(202002);广东省烟草专卖局(公司)科技项目(粤烟科项 201910)
作者单位
邵秀红,马柱文,袁清华,李集勤,潘晓英,黄振瑞  
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中文摘要:
      【目的】利用 高效液相色谱法(HPLC)检测繁种用烟草绿原酸、芦丁、茄尼 醇等重要活性成分 的含量差异,为烟草种质资源的有效开发利用提供参考。【方法】采用 HPLC 法测定 17 份繁种用烟草种质资源 成熟期叶片中绿原酸、芦丁、茄尼醇的含量,色谱条件为:Agilent 1260 色谱仪,色谱柱为 VP-ODS,流速 1.0 mL/min,柱温 35 ℃,检测器为 1260 VWD。【结果】6 种绿原酸异构体中,以绿原酸含量最高,在 17 份种质 资源中均有检测到,且以 S16 打宾柳叶烟晒烟最高,其次是隐绿原酸和新绿原酸含量,而异绿原酸 A、异绿原 酸 B 和异绿原酸 C 的含量与前 3 种异构体相比,总体偏低;芦丁含量以 NC347 烤烟最高(46.456 g/kg),其次 是 S19 大肩叶(7.782 g/kg)和 S17 大都青梗晒烟(6.250 g/kg),云烟 87 烤烟最低(1.206 g/kg);茄尼醇含量 以 NC347 烤烟最高(44.913 g/kg),其次是 K326 烤烟(11.140 g/kg)、S17 大都青梗晒烟(9.844 g/kg),黄坑 7 号 -1 烤烟最低(2.793 g/kg)。【结论】17 份烟草种质资源中芦丁和茄尼醇的含量与绿原酸相比总体较高,且 均以 NC347 烤烟最高。6 种异构体中含量最高的是绿原酸,且以 S16 打宾柳叶烟晒烟最高。各繁种用烟草种质 资源在现蕾后未打顶,这可能对绿原酸有较大影响。
英文摘要:
      【Objective】The contents of important active ingredients such as chlorogenic acid, rutin, and solanesol in different tobacco varieties for propagating were detected by HPLC method with a view to providing references for the effective development and utilization of tobacco germplasm resources.【Method】The contents of chlorogenic acid, rutin, and solanesol in leaves of 17 tobacco germplasm resources for propagating at mature stage were determined by HPLC. The chromatographic conditions were as follows: chromatograph was Agilent 1260, chromatographic column was VP-ODS, flow rate was 1.0 mL/min, the column temperature was 35℃ and the detector was 1260 VWD.【Result】The content of chlorogenic acid was the highest among the six isomers, and was detected in all 17 germplasm resources, and that of S16 Dabinliuyeyan sun-cured tobacco was the highest, followed by cryptochlorogenic acid and neochlorogenic acid. Compared with the first three isomers, the content of isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C was generally lower. The content of rutin in NC347 flue-cured tobacco was the highest(46.456 g/kg), followed by S19 Dajianye (7.782 g/kg)and S17 Daduqinggeng(6.250 g/kg)sun-cured tobaccos, and that of Yunyan 87 flue-cured tobacco was the lowest(1.206 g/kg). The content of solanesol in NC347 flue-cured tobacco was the highest(44.913 g/kg), followed by K326 flue-cured tobacco(11.140 g/kg)and S17 Daduqinggeng air-cured tobacco(9.844 g/kg), and that of Huangkeng No.7-1 flue-cured tobacco was the lowest(2.793 g/kg).【Conclusion】Among the 17 tobacco germplasm resources, the content of rutin and solanol was higher than that of chlorogenic acid, and the highest level was found in NC347 flue-cured tobacco. The content of chlorogenic acid was the highest among the six isomers, among which the S16 Dabinliuyeyan suncured tobacco had the highest content. The tobacco germplasm resources for propagating did not topping after the appearance of flower-buds, which may have a greater effect on chlorogenic acid.
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