| 韩福光 1
，王 珺 1，2，林秋露 1
，王 茜1，2.微绿球藻 (Nannochloropsis oculata培养基的优化[J].广东农业科学,2019,46(4):109-115 |
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微绿球藻 (Nannochloropsis oculata培养基的优化 |
| Optimization of the Culture Medium of Nannochloropsis oculata |
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| DOI：10.16768/j.issn.1004-874X.2019.04.016 |
| 中文关键词: 微绿球藻 培养基 优化 生长 影响 |
| 英文关键词: Nannochloropsis oculata culture medium optimization growth effects |
| 基金项目:海南省重点研发计划项目 (ZDYF2016087，ZDYF2016085)；海南省自然科学基金 (313039) |
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| 中文摘要: |
| 【目的】微绿球藻 (Nannochloropsis oculata) 是一种优良的单细胞饵料微藻，广泛应用于水产养殖。
优化微绿球藻培养基，以期提高其生长速率，降低生产成本。【方法】以宁波大学 3# 配方为基础微藻培养液，
以乙酸钠为碳源，硝酸钾、尿素和氯化铵为氮源，磷酸二氢钾和磷酸二氢钠为磷源，硫酸亚铁和柠檬酸铁为铁源，
通过单因子和正交试验，研究了碳、氮、磷、铁、维生素 B1
（VB1）和 B12（VB12）等主要营养元素对微绿球藻生长、
繁殖的影响。【结果】获得了以天然海水为基础的微绿球藻优化培养基：3 g/L CHCOONa、20 mg/L NH4CL-N、
2 mg/L KH2PO4-P、3 mg/L FeSO4-Fe、0.05 mg/L VB1 和 0.005mg/L VB12；采用优化培养基与宁波大学 3# 培养基对
比培养微绿球藻，结果表明 , 培养 2 ～ 6 d，优化培养基收获微绿球藻的生物量 ( 细胞密度 ) 比宁波大学 3# 培养
基的分别提高了 2.21、2.55、2.30、1.97、1.7 倍；培养 6 d，优化培养基中微绿球藻收获的生物量 ( 细胞密度 ) 达
到 1.74×107 个 /mL, 是宁波大学 3# 培养基的 1.7 倍。【结论】优化培养基极显著地提高了微绿球藻的生物量，
是微绿球藻的良好培养基。 |
| 英文摘要: |
| 【Objective】Nannochloropsis oculata is a kind of excellent single-celled diet microalgae, and widely
used in aquaculture.The culture medium of Nannochloropsis oculata was optimized in order to improve its growth rate and
reduce the production costs. 【Method】Taking Ningbo University 3# Formula as the basic microalgae culture medium,
with CHCOONa as carbon source, KNO3, (NH2)2CO and NH4CL as nitrogen source, KH2PO4 and NaH2PO4 as phosphorus
source, FeSO4 and FeC6H5O7 as iron source, the effects of different nutrient sources such as carbon, nitrogen, phosphorus,
iron,Vitamin B1 and Vitamin B12 on the growth and propagation of N. oculata were analyzed by one-factor experiment
and orthogonal test. 【Result】The optimized culture medium based on nature sea water was formulated as follows: 3 g/
L CHCOONa, 20 mg/L NH4Cl-N, 2 mg/L KH2PO4-P, 3 mg/L FeSO4-Fe, 0.05 mg/L Vitamin B1 and 0.005 mg/L Vitamin
B12. The N.oculata was cultured on optimized medium and Ningbo University 3# medium and the results showed that the
biomass (cell density) of N. oculata cultured on optimized medium for 2-6 days was 2.21, 2.55, 2.30, 1.97 and 1.7 times higher than that of Ningbo University 3# Medium respectively. By the end of the sixth day, the biomass (cell density) of N.
oculata on the optimized medium was up to 1.74×107 cell/mL, which was 1.7 times higher than that on Ningbo University
3# medium. 【Conclusion】The optimized medium extrenely enhamced the biomass of N. oculata and it was a good medium
for N. oculata. |
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