文章摘要
古广武 1 ,董辰韵 1 ,钟伟强 2,张安强 2,林雅铃 1.聚硅氧烷 - 聚丙烯酸酯季铵盐对 水稻纹枯病菌细胞膜的影响[J].广东农业科学,2019,46(7):92-99
聚硅氧烷 - 聚丙烯酸酯季铵盐对 水稻纹枯病菌细胞膜的影响
Effect of Polysiloxane-polyacrylate QuaternaryAmmonium Salt Block Copolymer onCell Membrane of Rhizoctonia solani
  
DOI:10.16768/j.issn.1004-874X.2019.07.014
中文关键词: 聚硅氧烷 - 聚丙烯酸酯季铵盐  嵌段共聚物  水稻纹枯病菌  细胞膜  渗透性  脂质过氧化
英文关键词: polysiloxane-polyacrylate quaternary ammonium salt  block copolymer  Rhizoctonia solani  cell membrane  permeability  lipid peroxidation
基金项目:广州市科技计划项目(201704020084,201803020015);国家自然科学基金(31772202)
作者单位
古广武 1 ,董辰韵 1 ,钟伟强 2,张安强 2,林雅铃 1 1. 华南农业大学材料与能源学院广东 广州 510642 2. 华南理工大学材料科学与工程学院广东 广州 510641 
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中文摘要:
      【目的】研究聚硅氧烷 - 聚丙烯酸酯季铵盐嵌段共聚物(Six-Q5)对水稻纹枯病菌(Rhizoctonia solani)细胞膜的影响,为水稻纹枯病的防治提供新思路。【方法】通过 PI 染色后在微分干涉显微镜下观察菌 丝形态表征 Six-Q5 对水稻纹枯病菌细胞膜完整性的影响,通过考马斯亮蓝法、蒽酮 - 硫酸法及电导率法表征 Six-Q5 对水稻纹枯病菌细胞膜渗透性的影响,利用丙二醛(MDA)试剂盒测定丙二醛的含量变化表征 Six-Q5 对 水稻纹枯病菌细胞膜脂质过氧化的影响。【结果】经过 Six-Q5 处理的菌丝显微镜下可观测到荧光亮斑,菌丝培 养液中的蛋白质、碳水化合物和电解质变化均明显区别于对照,水稻纹枯病菌菌丝及菌丝培养液中的 MDA 含 量均随着药物浓度的增加而增加,且随着 PDMS 嵌段长度的不同,其对水稻纹枯病菌菌丝细胞膜的影响也有所 差别。【结论】实验结果表明,Six-Q5 破坏了水稻纹枯病菌细胞膜的完整性,改变了细胞膜的渗透性及细胞膜 的结构功能完整性;且随着 Six-Q5 嵌段长度增加,其影响更加显著。细胞膜是 Six-Q5 抑制水稻纹枯病菌一个作 用靶点。
英文摘要:
      【Objective】The study was to investigate the effect of polysiloxane-polyacrylate quaternary ammonium salt block copolymer (Six-Q5) on cell membrane of Rhizoctonia solani, and to provide a new idea for the control of rice sheath blight.【Method】The effects of Six-Q5 on cell membrane integrity of R. solani were observed under differential interference microscopy after PI staining. The effects of Six-Q5 on cell membrane permeability of R. solani were characterized by Coomassie brilliant blue method, anthrone-sulfuric acid method and conductivity method, respectively. Malondialdehyde (MDA) kit was used to determine the content of MDA to characterize the effect of Six-Q5 on lipid peroxidation of cell membrane of R. solani.【Result】Under the fluorescence microscope, the mycelia treated with Six-Q5 are observed with fluorescent bright spots. It is found that the protein, carbohydrate and electrolyte contents in the mycelium culture medium of the treatment are significantly different from those of the control. The MDA content of mycelia and culture medium in the treatment increased with the increas of drug concentration. With the difference of PDMS block length, its influence on the R. solani mycelia membrane is also different.【Conclusion】The results show that Six-Q5 destroy the integrity of cell membrane, chang the permeability of cell membrane and the structural and functional integrity of cell membrane. Moreover, with the increase of the block length of Six-Q5, the effect is more pronounced. Cell membrane is a target of Six-Q5 for inhibiting rice sheath blight.
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