| 丁 玮1
，吕成群 1
，黄宝灵1
，李昆龙 2，陈振飞 2，任 涵 1
，康 凯 1.泡桐根瘤内生细菌的分离及初步鉴定[J].广东农业科学,2019,46(1):36-42 |
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泡桐根瘤内生细菌的分离及初步鉴定 |
| Isolation and Preliminary Identification of Endophytic Bacteria in the Root Nodule of Paulownia |
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| DOI：10.16768/j.issn.1004-874X.2019.01.006 |
| 中文关键词: 泡桐 根瘤内生细菌 生理生化特性 16S rDNA 全序列分析 |
| 英文关键词: Paulownia nodule endophytic bacteria physiological and biochemical characteristics 16S rDNA complete sequence analysis |
| 基金项目:广西林业科技项目（桂林科研 2015 第 25 号） |
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| 摘要点击次数: 1985 |
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| 中文摘要: |
| 【目的】为了探究非豆科植物泡桐与微生物共生固氮体系，对泡桐根瘤内生细菌的分离培养条件
及其系统发育地位进行初步研究。【方法】对从广西钦州市采集的泡桐根瘤上分离纯化得到的根瘤内生细菌，
进行传统生理生化研究和 16S rDNA 系统发育分析。【结果】经分离纯化得到 9 株根瘤内生细菌，大部分菌株
能较好地利用 8 种碳源；78% 的菌株能利用铵盐，89% 的菌株能利用硝酸盐；所有的供试菌株在 3- 酮基乳糖
和淀粉水解试验中呈阴性；89% 的菌株能利用柠檬酸盐；B.T.B 反应除菌株 PG-7 产碱外，其余都产酸；33%
的菌株能使硝酸盐还原；有 56% 的菌株能在牛肉膏蛋白胨培养基上生长良好，多数菌株具备豆科植物根瘤菌的
一般生理生化特征。通过 16S rDNA 全序列分析，确定 9 株供试菌株可分为 4 个菌属，其中菌株 PG-1 和 PG-2
共属于土壤杆菌属（Agrobacterium），PG-3、PG-5、PG-6、PG-8 和 PG-9 等 5 株菌株共属于根瘤菌属（Rhizobium），
PG-4 隶属于草螺菌属（Herbaspirillum），PG-7 隶属于伯克氏菌属（Burkholderia）。【结论】分离自同一寄主
的根瘤内生细菌其生理生化特性方面存在较大的差异性，同时表现出了遗传的多样性。 |
| 英文摘要: |
| 【Objective】In order to explore the symbiotic nitrogen fixation system of non-leguminous plants
Paulownia and microorganism, a preliminary study was carried out on isolated culture conditions and the phylogenetic
position of endophyic bacteria in Paulowia root nodules.【Method】The endophytic bacteria isolated and purified from
the root nodules of Paulownia collected from Qinzhou of Guangxi Province were subjected to traditional physiological
and biochemical studies and 16S rDNA phylogenetic analysis.【Result】In the nine strains of endophytic bacteria
isolated and purified, most of the bacterial strains could make good use of 8 carbon sources; 78% of the bacterial strains
could use ammonium salt, and 89% of the bacterial strains could use nitrate. All tested bacterial strains were negative
in the 3-ketolactose and starch hydrolysis test; 89% of the strains could use citrate. Except that the bacterial strains
PG-7 produced alkali, the rest of bacterial strains produced acid in the B.T.B reaction; 33% of the strains could reduce
nitrate; 56% of the strains grew well on beef extract peptone medium, and most strains have the general physiological
and biochemical characteristics of leguminous rhizobium after identification. According to 16S rDNA complete sequence analysis, nine bacterial strains can be divided into four bacteria genera, among which the bacterial stains PG-1 and PG-2
belong to Agrobacterium, PG-3, PG-5, PG-6, PG-8 and PG-9 belong to Rhizobium, PG-4 belongs to Herbaspirillum and PG-7
belongs to Burkholderia.【Conclusion】The endophytic bacteria isolated from the same root nodule have great differences in
their physiological and biochemical characteristics of endogenous bacteria , and showed genetic diversity. |
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