| 陆 济，游春平，董章勇.花生青枯病生防细菌的筛选与鉴定[J].广东农业科学,2019,46(2):94-98 |
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花生青枯病生防细菌的筛选与鉴定 |
| Screening and Identification of Biocontrol Bacteria Against Bacterial Wilt of Peanuts |
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| DOI：10.16768/j.issn.1004-874X.2019.02.014 |
| 中文关键词: 花生 青枯病 生物防治 筛选 绿脓假单胞杆菌 |
| 英文关键词: peanut bacterial wilt biological control screening pseudomonas aeruginosa |
| 基金项目:广东省普通高校特色创新项目（2016KTSCX060） |
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| 摘要点击次数: 1795 |
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| 中文摘要: |
| 【目的】筛选花生青枯病的生物防治菌株。【方法】利用平板对峙法筛选对花生青枯病有拮抗
作用的生物防治细菌菌株，利用盆栽试验筛选防治效果好的细菌菌株。【结果】从根际土壤中分离的 300 余
株细菌中筛选获得 7 株对花生青枯病有拮抗作用的菌株。其中菌株 Ab、A27 和 A38 的抑菌直径超过 10 mm，
且其分泌物对该菌有抑制效果。将这 3 个菌株进一步进行盆栽试验，结果表明 A38 的防治效果最好，达到
52.72%。通过生理生化及分子生物学鉴定，将 A38 菌株鉴定为绿脓假单胞杆菌（Pseudomonas aeruginosa）。【结
论】筛选到一种花生青枯病菌拮抗效果较好的生防细菌，鉴定为绿脓假单胞杆菌。 |
| 英文摘要: |
| 【Objective】To Screen the biocontrol bacterial strains against bacterial wilt of peanuts. 【Method】
The biocontrol bacterial strains which have antagonistic effects on bacterial wilt of peanuts were screened by plate
confrontation method and the bacterial strains with good control effect were screened by pot experiment.【Result】Seven
strains with antagonistic effects on bacterial wilt of peanuts were screened from over 300 bacteria strains isolated from
rhizosphere soil. Among them, strains Ab, A27 and A38 had a bacterial inhibition diameter of more than 10 mm, and
their secretions had an inhibitory effect on the bacteria. The three strains were further subjected to pot experiment, and
the results showed that A38 got the best control effect, reaching 52.72%. The strain A38 was identified as Pseudomonas
aeruginosa by physiological, biochemical and molecular biological identification.【Conclusion】A biocontrol bacterium
with good antagonistic effect against bacterial wilt of peanuts was screened and identified as Pseudomonas aeruginosa. |
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