| 陆敏，张绍岩，于宏伟，宋淑宁.绿茶毛尖中有机酸的高效液相色谱分析[J].广东农业科学,2012,39(2):86-87 |
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绿茶毛尖中有机酸的高效液相色谱分析 |
| Analysis of organic acids in Maojian green tea by high performance liquid chromatography |
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| DOI： |
| 中文关键词: 绿茶 有机酸 高效液相色谱法 |
| 英文关键词: green tea organic acids high performance liquid chromatography |
| 基金项目: |
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| 摘要点击次数: 1772 |
| 全文下载次数: 705 |
| 中文摘要: |
| 建立了测定绿茶毛尖中有机酸的高效液相色谱方法。应用反相C18色谱柱，0.05 mol/L KH2PO4（pH=2.7）为流动相，流速为0.6 mL/min，检测波长为210 nm，柱温为30℃，使绿茶中7 种有机酸得到很好地分离。各有机酸在线性范围内呈良好的线性关系，回收率在95.6%~102.5%之间。该方法操作简单、快速、准确度高，适用于绿茶中有机酸含量的测定。 |
| 英文摘要: |
| Detectionation of organic acids in Maojian green tea by HPLC was studied. A reversed-phase C18 column was used with the mobile phase of 0.05 mol/L KH2PO4 （pH=2.7）at 0.6 mL/min and detection wavelength at 210 nm and column temperature of 30℃，it can be seperated well for the seven organic acids in MaoJian green tea. The organic acids had good linear in the linear range with
recoveries between 95.6%~102.5%. The method was simple，rapid and with high accuracy which was suitable for determination of organic acids in green tea. |
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