| 王岩，杨明凡，崔保安，阴晓霞，邓永欢，闫艺克，王汉邦.鸭瘟病毒河南株gI基因的克隆与测序[J].广东农业科学,2012,39(3):139-140 |
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鸭瘟病毒河南株gI基因的克隆与测序 |
| Clone and sequencing of gI gene of duck plague virus Henan strain |
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| DOI： |
| 中文关键词: 鸭瘟病毒 gI基因 克隆 测序 |
| 英文关键词: DPV gI gene cloning sequencing |
| 基金项目: |
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| 摘要点击次数: 1829 |
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| 中文摘要: |
| 参考GenBank 中鸭瘟病毒gI 基因序列设计并合成引物，以鸭瘟病毒河南分离株DNA 为模板进行PCR 扩增，将扩增片段克隆至PGEM-T 载体上，得到含gI基因重组质粒。经酶切鉴定，并对重组阳性质粒进行序列测定。结果表明，获得的片段含鸭瘟病毒gI 基因，全长1 089 bp，与已报道的其他疱疹病毒gI 基因具有较高的同源性，编码432 个氨基酸，蛋白分子质量为39.7ku、等电点（PI）为6.06。 |
| 英文摘要: |
| According to published duck plague virus (DPV)gI gene sequences, a pair of specific primers was designed and synthesized. The genomic DNA of DPV Henan strain, which was isolated from Henan, was used as template.The PCR product cloned into the PGEM-T vector. The gene was sequenced and a complete open reading frame was found by bioinformatics tools of ORF Finder and Blastn. Bioinformatics analysis showed that the ORF of 1 089 bp encoding gI gene and encoding 362 amino acids was obtained. The protein molecular weight was 39.7 ku,and its isoelectric point PI was 6.060. |
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