| 刘正华，杨娟，李拥军，李谦，郑飞腾，李盛安，冯敏铃.高效液相色谱-串联质谱法测定猪肝中硝基呋喃类代谢物残留[J].广东农业科学,2012,39(4):87-89 |
|
查看全文
HTML
高效液相色谱-串联质谱法测定猪肝中硝基呋喃类代谢物残留 |
| Determination of nitrofuran metabolite residues in liver by liquid chromatography tandem mass spectrometry |
| |
| DOI： |
| 中文关键词: 高效液相色谱-串联质谱 猪肝 硝基呋喃类代谢物 残留 |
| 英文关键词: HPLC-MS/MS liver nitrofuran metabolite residue |
| 基金项目: |
|
| 摘要点击次数: 2058 |
| 全文下载次数: 842 |
| 中文摘要: |
| 建立了呋喃唑酮代谢物AOZ、呋喃西林代谢物SEM尧、呋喃妥因代谢物AHD、呋喃它酮代谢物AMOZ 的高效液相色谱-串联质谱（HPLC-MS/MS）测定方法、样品经乙酸乙酯提取后，用Oasis HLB 固相萃取柱净化，以乙腈和甲酸-乙酸铵缓冲溶液为流动相，梯度洗脱，采用电喷雾正离子（ESI+）电离，多反应监测（MRM）模式检测，外标法定量。4种硝基呋喃类代谢物在0.5~20.0 ng/mL范围内均具有良好的线性关系，相关系数不低于0.9906，平均加标回收率为91.0%~101.5%，相对标准偏差均小于8%。该方法具有准确、灵敏、高效、环保，适用于猪肝中多种硝基呋喃类代谢物的同时测定。 |
| 英文摘要: |
| A HPLC-MS/MS method was developed for determination of 4 nitrofuran metabolite residues in liver. The sample was extracted with ethyl acetate and purified by Oasis HLB solid -phase extraction column, then detected by high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Acetonitrile and formic acid-ammonium acetate buffer were used as mobile phase by gradient elution. The analysis of target compounds was carried out by HPLC-MS/MS under positive electrospray ionization (ESI+) and multiple reaction monitoring (MRM) mode. The calibration curves for 4 nitrofuran metabolite residues all had good linear relationships with correlation coefficients not less than 0.9906. The spiked recoveries were ranged from 91.0% to 101.5% with relative standard deviations less than 8%. It was an effective method which fit for the nitrofuran metabolite residues analysis and has high accuracy, precision and sensitivity. |
|
查看/发表评论 下载PDF阅读器 |
