| 傅冰，俞汇颖，魏云林，季秀玲，郑维桃.鸡蛋清中3种蛋白质的连续化提取工艺初步研究[J].广东农业科学,2013,40(1):92-95 |
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鸡蛋清中3种蛋白质的连续化提取工艺初步研究 |
| Preliminary study on the separation of lysozyme, ovotransferrin and ovalbumin from egg white |
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| DOI： |
| 中文关键词: 鸡蛋清 溶菌酶 卵白蛋白 卵转铁蛋白 离子交换层析 分离 |
| 英文关键词: egg white lysozyme ovalbumin ovotransferrin anion-exchange chromatography separation |
| 基金项目:丽水市科技局校企项目(20080410) |
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| 中文摘要: |
| 研究鸡蛋清中溶菌酶、卵转铁蛋白和卵白蛋白的分离提取工艺遥采用硫酸铵沉淀和离子交换层析分离3种蛋白质，并用聚丙烯酰胺凝胶电泳（SDS-PAGE）检测其纯度。结果显示，层析纯化后SDS-PAGE 检测其纯度达到电泳纯，溶菌酶的比活由144.13 U/mg 提高到2235 U/mg，纯化倍数达15倍，回收率为15.76%；卵转铁蛋白抑菌率为48.84%。该方法分离溶菌酶、卵转铁蛋白和卵白蛋白的工艺路线简单、快捷、成本低，适用于工业生产。 |
| 英文摘要: |
| Separation processing of lysozyme, ovotransferrin and ovalbumin from egg white proteins was studied. The proteins were
separated by ammonium sulfates and anion-exchange chromatography. Purity of the proteins was assayed by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE). The results showed that the proteins were electrophoresis-pure. The specific activity of lysozyme was increased from 144.13 U/mg to 2 235 U/mg, and purification factor was 15-fold. Lysozyme recoveries were estimated to be 15.76%. Antibacterial probability of ovotransferrin was 48.84%. The procedure for separation lysozyme, ovotransferrin and ovalbumin from egg white was simple, fast, low-cost and suitable for industry. |
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