| 郭芹，王吉德，李雪萍，陈维信，吴斌.一氧化氮处理对采后番木瓜果实乙烯生物合成的影响[J].广东农业科学,2013,40(3):75-78 |
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一氧化氮处理对采后番木瓜果实乙烯生物合成的影响 |
| Effects of nitric oxide treatment on ethylene biosynthesis in harvested papaya fruit |
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| DOI： |
| 中文关键词: 番木瓜 一氧化氮 乙烯合成 ACC 合成酶 ACC 氧化酶 |
| 英文关键词: papaya nitric oxide ethylene synthesis ACC synthase ACC oxidase |
| 基金项目:国家自然科学基金-广东省联合基金项目(U0631004)；广东省自然科学基金团队项目（06200670）；科技部支撑计划项目（2011BAD24B02-4） |
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| 摘要点击次数: 1699 |
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| 中文摘要: |
| 为了解一氧化氮（NO）处理对番木瓜果实乙烯生物合成的影响，采用60μL/L NO 熏蒸处理采收成熟度为果皮浅绿并微带黄色痕迹的番木瓜果实3 h，然后在20℃和相对湿度为85%条件下贮藏20 d。研究NO 对番木瓜乙烯、1-氨基环丙烷-1-羧酸（ACC）、丙二酰-1-氨基环丙烷-1-羧酸（MACC）、ACC 合成酶（ACS）和ACC 氧化酶（ACO）及CpACS2 和CpACO1基因表达的影响。结果表明，NO 处理降低了番木瓜果实乙烯释放量、ACO 的活性及CpACO1基因的表达，导致贮藏过程果实ACC 和MACC的积累，但对ACS 的活性及CpACS2基因的表达无显著抑制作用。 |
| 英文摘要: |
| Effects of nitric oxide (NO) on ethylene biosynthesis in papaya fruit were investigated. Papaya fruit harvested at light green with some hint of yellow at the blossom end, were first fumigated with 60 μL/L NO for 3 h, then stored at 20℃ with 85% relative humidity (RH) for 20 days. Changes in the levels of ethylene, 1 -aminocyclopropane -1 -carboxylic acid (ACC), 1 - malonylaminocyclopropane-1-carboxylic acid (MACC), as well as the activities of ACC synthase (ACS), ACC oxidase (ACO) and the expression of CpACS2 and CpACO1 genes were measured during papaya ripening. The results showed that NO treatment inhibited ethylene production, ACO activity and the expression of CpACO1 gene, leading to the accumulation of ACC and MACC during storage.However, ACS activity and the expression of CpACS2 gene were not suppressed significantly by NO treatment. |
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