| 夏月恒，章群，高志远，陈德荫，李贵生.中国近海鮸鱼遗传多样性的细胞色素b全序列分析[J].广东农业科学,2013,40(3):101-105 |
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中国近海鮸鱼遗传多样性的细胞色素b全序列分析 |
| Genetic diversity analysis of Miichthys miiuy in coastal waters of China based on complete Cytochrome b gene sequences |
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| DOI： |
| 中文关键词: 鮸鱼 细胞色素b 遗传变异 |
| 英文关键词: Miichthys miiuy cytochrome b genetic variation |
| 基金项目:国家自然科学基金（41071034，30770415）；科研培育基金（21611426） |
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| 摘要点击次数: 1504 |
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| 中文摘要: |
| 分析了中国近海连云港、舟山和福州38 尾鮸鱼线粒体细胞色素b（Cytb）基因的1140 bp序列，共检测到39 个变异位点和5个简约信息位点；共有27 个单倍型，总的单倍型多样性为0.960±0.020，核苷酸多样性为0.00271±0.00030，表现为高单倍型多样性和低核苷酸多样性；Tajima's 中性检验为负值且显著（-2.36010，P<0.05），在简约性网络图中单倍型呈星状分布，核苷酸不配对分析呈单峰曲线，表明中国近海鮸鱼经历过种群扩张，推测扩张年代约为8万年前。在邻接树和简约性网络图中不同地理来源的单倍型交错分布，群体间有较低的Fst值（-0.0190~-0.0081）和较高的Nm 值(-62.53~-26.82)，分子方差分析（群体间-1.12%）表明群体间未出现明显的遗传分化，可作为一个管理保护单位，推测鮸鱼生活史中存在一定规模的洄游以及扩张后的群体尚未在迁移与漂变间达到平衡是未检测到显著地理和谱系结构的主要原因。 |
| 英文摘要: |
| Complete sequences of mtDNA Cytochrome b gene (1 140 bp) of 38 individuals of Miichthys miiuy collected in
Lianyungang, Zhoushan and Fuzhou along coastal waters of China were analyzed, of which 27 haplotypes were defined by 39 variable sites and 5 parsimony-informative sites. The global haplotype diversity (Hd) and nucleotide diversity (Pi) were 0.960±0.020 and 0.00271± 0.00030 respectively, which showed high haplotype diversity and low nucleotide diversity. Neutral test of Tajima’s (-2.36010), star-like statistical parsimony cladogram and mismatch distribution revealed a population expansion at about 80 thousand years in Miichthys miiuy. Low genetic differentiated index (Fst=-0.0190~-0.0081) and high gene flow (Nm=-62.53~-26.82), intertwined haplotypes of various geographic populations in Neighbor-joining and TCS tree, and analysis of molecular variance (AMOVA, -1.12%) among populations showed no significant population differentiation,and the three populations could be protected as a single management unit, which were inconsistent with conventional partitions of geographical population. A certern degree of migration in the life history and insufficient time to attain a equilibrium after a large range population expansion may be attributed to account for the lack of genetic structure. |
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