| 徐亚军，赵龙飞，代猛，阮文静，白莉敏，张广月，步玲玲，周佩佩，张嫚.药用植物金银花内生细菌的分离和特性研究[J].广东农业科学,2013,40(3):115-118 |
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药用植物金银花内生细菌的分离和特性研究 |
| Isolation and characteristic of endophytic bacteria in medicinal plant Flos lonicerae |
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| DOI： |
| 中文关键词: 金银花 内生细菌 生理生化特性 芽孢杆菌属 |
| 英文关键词: Flos lonicerae endophytic bacteria physiological and biochemical characteristics Bacillus |
| 基金项目:国家自然科学基金(U1204301)；河南省高等学校青年骨干教师计划项目(2012GGJS166)；商丘师范学院大学生创新实验计划项目(2011DXS03) |
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| 摘要点击次数: 1844 |
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| 中文摘要: |
| 以药用植物金银花为材料，对其根部、茎部、叶片不同部位的内生细菌进行分离纯化，对细胞特征采用革兰氏染色、芽孢染色法进行研究，结合菌落特征和生理生化特性将其内生细菌初步分为第1类型和第2类型两大类。采用稀释涂布法对金银花不同部位分别进行可培养内生细菌计数。结果表明，内生菌的总体数量分布为根部>叶片>茎部，说明内生细菌在金银花组织中的分布因部位不同而不同。基于细胞染色特征、显微观察结果和培养特性，内生菌中第1类型符合芽孢杆菌属(Bacillus)的特点，第2类型具有类芽孢杆菌属(Paenibacillus)的特征。 |
| 英文摘要: |
| Taking medicinal plant Flos Lonicerae as experimental material, endophytic bacteria of different parts of root, stem and
leaf were isolated and purified, cell features were determined by Gram dye and Spore dye methods. They were preliminary classified into two types （first type and second type） combined clony characteristic, physiological and biochemical characteristics. Microbes were counted by dilution spread method for different parts of F. lonicerae. Results indicated that distribution of endophytic bacteria total number in root was the most, leaf was second and stem was the least, it suggestted that distribution difference of endophytic bacteria in F. lonicerae was in accordance with different parts. Based on dye features, microscopic observation results and cultive characteristics, endophytic bacterial first type was accorded with genus Bacillus, and second type was accorded with characteristics of genus Paenibacillus. |
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