| 周金忠，潘学军，黄玫，张文娥.不同供硼水平对野生毛葡萄试管苗生长的影响[J].广东农业科学,2013,40(4):26-29 |
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不同供硼水平对野生毛葡萄试管苗生长的影响 |
| Effect of differential boron levels on growth of wild Vitis heyneana seedlings in vitro |
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| 基金项目:贵州省优秀青年科技人才培养对象专项(黔科合人字[2009]02号) |
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| 中文摘要: |
| 以4个野生毛葡萄单株为试材，研究了5个（0、1.1、3、6、9 mg/L）供硼水平下毛葡萄试管苗的生长差异，为毛葡萄单株对低硼和高硼的耐性提供初步筛选结果。结果表明，缺硼和高硼均影响毛葡萄的生长，与对照（1.1 mg/L）相比，缺硼导致毛葡萄株高显著下降，但对根系生长影响较小，总根长、平均根直径、总根表面积和总根体积与对照差异不显著；花溪-4对低硼胁迫的耐性最强。高硼胁迫显著抑制毛葡萄地上部生长，且硼浓度越高，抑制效果越明显；3~6 mg/L的高硼胁迫对花溪-4、花溪-9和农院-11单株的根系生长表现出促进效果，但当硼浓度达到9 mg/L时，根系生长受到明显抑制。福泉-1根系生长对高硼胁迫较为敏感，3个高硼水平下总根长、平均根直径、总根表面积和总根体积均低于对照，且随着硼浓度的升高，抑制程度不断加大。本研究可初步判断花溪-4的耐低硼能力最强，而福泉-1对高硼胁迫的耐性最差。 |
| 英文摘要: |
| For the purpose of providing preliminary results about effect of low boron and high boron levels on four individuals of Vitis heyneana, the growth characteristics of wild V. heyneana seedlings in tissue culture conditions was studied under 5 boron levels (0,1.1, 3, 6 and 9 mg/L) . The results showed that high or low boron stresses all affected the growth of V. heyneana seedling, low boron levels in the medium significantly decreased the plant height of the V. heyneana, conversely , the root growth wasn爷t effect. No significant changes in total root length, average root diameter, total root surface-area and total root volume. Huaxi-4 was the most tolerant to low boron stress. The plant height was inhibited by high levels boron. The inhibited effect was more and more obvious with increased concentration of boron. The root growth of three individuals (Huaxi-4, Huaxi-9 and Nongyuan-11) was accelerated with the boron in concentration of 3~6 mg/L, but the root growth was inhibited obviously when the boron level was up to 9 mg/L. The root growth of Fuquan-1 was the most sensitive to high boron stress. Total root length, average root diameter, total root surface-area and total root
volume under three high boron stress all decreased. Along with the higher boron concentration, the inhibitor effect was more obvious. According these results, the Huaxi-4 was more tolerant to the low boron stress. On the contrary, the Fuquan-1 was the most sensitive to the high boron stress. |
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