| 邓添，谢少雄，董世超，王承恩，李克克，陈剑锋，杨妙贤.臭马比木茎段培养中污染控制和防褐化研究[J].广东农业科学,2013,40(6):25-28 |
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臭马比木茎段培养中污染控制和防褐化研究 |
| Contamination control and browning prevention during tissue culture of stems of Nothapodytes nimmoniana |
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| DOI： |
| 中文关键词: 臭马比木 组织培养 污染 褐化 |
| 英文关键词: Nothapodytes nimmoniana tissue culture contamination browning |
| 基金项目:广东省大学生创新实验项目（1134710024）；仲恺农业工程学院基础生物学与生物技术实验教学示范中心开放基金（IP2010003） |
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| 中文摘要: |
| 研究了臭马比木（Nothapodytes nimmonian）组织培养过程中的防污染和防褐化问题。结果表明：在3~4月份取1 年生嫩茎进行诱导，外植体的污染率最低、为17.5%，褐化枯死率为39.2%，诱导率为43.3%，诱导效果最佳；在4个茎段部位中，半木质化茎段的诱导效果最好，诱导率为51.7%；低温预处理外植体对污染的控制效果不明显；0.1% HgCl2 8 min+50 mg/L 噻孢霉素30min 的消毒效果最好；在培养基中添加活性炭1.2 g/L+聚乙烯吡咯烷酮（PVP）2.0 g/L对外植体的防褐化效果最好，褐化率由对照的48.3%下降到25.0%。 |
| 英文摘要: |
| The prevention of contamination and browning during tissue culture of Nothapodytes nimmoniana was investigated. The
results showed that the lowest contamination rate was achieved with 1-year-old tender stem as explants in March and April, then the contamination rate was 17.5%, browning rate was 36.2% and induction rate was 43.3%. In the 4 parts of stems, the induction effect of semi-lignified stem segments was the best, its induction rate was 51.7%. Low temperature pretreatment of explants had no significant effect on contamination control. The sterilizing effect of “0.1% HgCl2 8 min + 50 mg/L Cefotaxime 30 min” on the stem explants was the optimum. The lowest browning rate of the explants was achieved with the addition of 1.2 g/L activated carbon plus 2.0 g/L polyvinylpyrrolidone (PVP) to the medium, then the browning rate was 25%. |
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